Hi Peng

nifti should be fine as input, but definitely don't use analyze. It doesn't preserve the direction cosines so once you convert to it you will never be sure which side of the image is the left hemim and which is the right.

I can't help with the 'can't locate MNI/Startup.pm ' error. Hopefully someone else can.

You will need some T1-weighted image with reasonably high res as input htough.

cheers
Bruce

On Wed, 9 Dec 2015, Peng Zan wrote:

Hi Bruce,
Thank you for your email! These dicom files are transferred from nifti
files, '.nii.gz' by xmedcon, and I think for this subject(R2261_S0283), they
are not mprage or flash scans and so far I do not have mprage or flash scans
for this subject. 

However, I do have mprage dicom files for other subjects in .img format,
which freesurfer cannot read(I attached two screenshots of my folder in case
you know by their names which files can work). Since I have most of my MRI
data in nifti and .img formats, it is worth the efforts to make these files
work in freesurfer. Do the input files have to be distributed into many
dicom slices or they can be in one file in .nii or .dcm? 

On the other hand, I also tested the my dicom files of subject R2105 (in
flash scans), and here comes the errors 'can't locate MNI/Startup.pm in
@INC...', and the 'log' file warns the input volume axes are not orthogonal.
(see 'recon-all.log' file attached). I think the input dicoms should not
have coordinates themselves, they are aligned and then assigned coordinates
by freesurfer, am I right? 

I am sorry for my verbose problems at this very beginning of using
freesurfer. 

Thank you very much! 

Sincerely,
Peng





On Wed, Dec 9, 2015 at 3:08 PM, Bruce Fischl <fis...@nmr.mgh.harvard.edu>
wrote:
      Hi Peng

      it looks like we can't read your input dicom. For example, the
      log says:
      reading from
/Users/Peng/Documents/MNE/MNE_analysis/MRI/R2261_S0283/m000-gm_A0136_N12518
      _S0283_20120817_P5716_averageCBF_normalized.dcm...
      Starting DICOMRead2()
      dcmfile =
/Users/Peng/Documents/MNE/MNE_analysis/MRI/R2261_S0283/m000-gm_A0136_N12518
      _S0283_20120817_P5716_averageCBF_normalized.dcm
      dcmdir = /Users/Peng/Documents/MNE/MNE_analysis/MRI/R2261_S0283
      WARNING: tag ImageNumber not found in
/Users/Peng/Documents/MNE/MNE_analysis/MRI/R2261_S0283/m000-gm_A0136_N12518
      _S0283_20120817_P5716_averageCBF_normalized.dcm
      WARNING: tag SeriesNumber not found in
/Users/Peng/Documents/MNE/MNE_analysis/MRI/R2261_S0283/m000-gm_A0136_N12518
      _S0283_20120817_P5716_averageCBF_normalized.dcm
      WARNING: tag image orientation not found in
/Users/Peng/Documents/MNE/MNE_analysis/MRI/R2261_S0283/m000-gm_A0136_N12518
      _S0283_20120817_P5716_averageCBF_normalized.dcm
      Ref Series No = 0
      Found 5 files, checking for dicoms
      WARNING: tag ImageNumber not found in
/Users/Peng/Documents/MNE/MNE_analysis/MRI/R2261_S0283/m000-gm_A0136_N12518
      _S0283_20120817_P5716_averageCBF_normalized.dcm

      where did that dicom file come from? And is it supposed to be a
      T1-weighted volume like an mprage or FLASH scan?

      cheers
      Bruce



      On Wed, 9 Dec 2015, Peng Zan
      wrote:

      > Dear freesurfer expert,
      > I am a student from University of Maryland, learning
      freesurfer for MNE
      > analysis recently. I have a perhaps silly problem and could
      not solve by
      > myself. The problem occurred as 'recon-all -s R2261_S0283
      exited with
      > errors...' when I was running 'recon-all -autorecon-all
      -subjid
      > R2261_S0283'. I tried several subjects and got the same error.
      I am
      > wondering whether you can look at the 'recon-all.log' file and
      > 'recon-all.error' file and help me figure out what the problem
      is and how to
      > solve it?
      >
      > Thank you very much! Looking forward to your reply!
      >
      > Sincerely,
      > Peng
      >
      >
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