Hi Joelle – I tested one of your dicoms and the gradient table that mri_convert 
reads from it works just fine. So you can just pass your dicoms to TRACULA and 
it’ll do the rest. No need for you to specify the gradient tables.

Best,
a.y

From: 
<freesurfer-boun...@nmr.mgh.harvard.edu<mailto:freesurfer-boun...@nmr.mgh.harvard.edu>>
 on behalf of Joëlle Ismay Rosanne Van Der Molen 
<joelle.vandermo...@etu.unige.ch<mailto:joelle.vandermo...@etu.unige.ch>>
Reply-To: Freesurfer support list 
<freesurfer@nmr.mgh.harvard.edu<mailto:freesurfer@nmr.mgh.harvard.edu>>
Date: Friday, March 31, 2017 at 7:44 AM
To: Freesurfer support list 
<freesurfer@nmr.mgh.harvard.edu<mailto:freesurfer@nmr.mgh.harvard.edu>>
Subject: Re: [Freesurfer] TRACULA tract reconstruction


​Hi Anastasia,


I sent the links for the dicom files to you and just want to make sure - did 
you receive them?


Best,


Joëlle

________________________________
De : 
freesurfer-boun...@nmr.mgh.harvard.edu<mailto:freesurfer-boun...@nmr.mgh.harvard.edu>
 
<freesurfer-boun...@nmr.mgh.harvard.edu<mailto:freesurfer-boun...@nmr.mgh.harvard.edu>>
 de la part de Yendiki, Anastasia 
<ayend...@mgh.harvard.edu<mailto:ayend...@mgh.harvard.edu>>
Envoyé : jeudi 30 mars 2017 15:20
À : Freesurfer support list
Objet : Re: [Freesurfer] TRACULA tract reconstruction

Hi Joëlle - Can you please send the filedrop upload to me too? Thanks!

a.y

________________________________
From: 
freesurfer-boun...@nmr.mgh.harvard.edu<mailto:freesurfer-boun...@nmr.mgh.harvard.edu>
 
[freesurfer-boun...@nmr.mgh.harvard.edu<mailto:freesurfer-boun...@nmr.mgh.harvard.edu>]
 on behalf of Joëlle Ismay Rosanne Van Der Molen 
[joelle.vandermo...@etu.unige.ch<mailto:joelle.vandermo...@etu.unige.ch>]
Sent: Thursday, March 30, 2017 7:27 AM
To: Freesurfer support list
Subject: Re: [Freesurfer] TRACULA tract reconstruction


​Hi Barbara,


Thank you for the tip, I managed to anonymize my dicom images. I uploaded them 
on filedrop. ​

The scanner used for the acquisition is a Siemens Prisma 3 Tesla.


Best,

Joëlle

________________________________
De : 
freesurfer-boun...@nmr.mgh.harvard.edu<mailto:freesurfer-boun...@nmr.mgh.harvard.edu>
 
<freesurfer-boun...@nmr.mgh.harvard.edu<mailto:freesurfer-boun...@nmr.mgh.harvard.edu>>
 de la part de Barbara Kreilkamp 
<bakk....@googlemail.com<mailto:bakk....@googlemail.com>>
Envoyé : mercredi 29 mars 2017 14:18
À : Freesurfer support list
Objet : Re: [Freesurfer] TRACULA tract reconstruction


Hi Joëlle,

Which scanner was used to acquire the images? I have used Osirix light in the 
past to anonymize my dicom images, maybe you'd like to try that?

Best wishes,

Barbara

On 29/03/2017 13:30, Joëlle Ismay Rosanne Van Der Molen wrote:

​Hi Anastasia,


Yes, I use dicom images as input for Tracula. I also specify the bvecs files, 
as Tracula returns an error if I try to run it with a configuration file that 
does not contain them.



Unfortunately, our dicom images are not anonymised, so I cannot provide them.


Best,


Joëlle


________________________________
De : 
freesurfer-boun...@nmr.mgh.harvard.edu<mailto:freesurfer-boun...@nmr.mgh.harvard.edu>
 
<freesurfer-boun...@nmr.mgh.harvard.edu><mailto:freesurfer-boun...@nmr.mgh.harvard.edu>
 de la part de Yendiki, Anastasia 
<ayend...@mgh.harvard.edu><mailto:ayend...@mgh.harvard.edu>
Envoyé : mercredi 22 mars 2017 15:40
À : Freesurfer support list
Objet : Re: [Freesurfer] TRACULA tract reconstruction

Hi Joëlle – What images do you pass as input to TRACULA? Do you pass the dicom 
itself, or a converted file? Happy to look at your dicom, if it’s anonymized.

a.y

From: 
<freesurfer-boun...@nmr.mgh.harvard.edu<mailto:freesurfer-boun...@nmr.mgh.harvard.edu>>
 on behalf of Joëlle Ismay Rosanne Van Der Molen 
<joelle.vandermo...@etu.unige.ch<mailto:joelle.vandermo...@etu.unige.ch>>
Reply-To: Freesurfer support list 
<freesurfer@nmr.mgh.harvard.edu<mailto:freesurfer@nmr.mgh.harvard.edu>>
Date: Wednesday, March 22, 2017 at 3:55 AM
To: Freesurfer support list 
<freesurfer@nmr.mgh.harvard.edu<mailto:freesurfer@nmr.mgh.harvard.edu>>
Subject: Re: [Freesurfer] TRACULA tract reconstruction

Hi Anastasia and Antonin,

I apologize for the late response.
Thank you very much for the explanations!

Regarding the gradient tables, I realized that I was indeed using the old 
version of mrtrix3, and was getting tables with a flipped x component (sign 
inversion), which corresponds to the bug that is highlighted in the blog link 
you sent me. After having updated mrtrix3, the tables now look correct.

What seems odd, however, is that in fact mri_convert outputs the same gradient 
tables as the old version of mrtrix3, that is, with a flipped x component.
I tried running tracula on one of our subjects using gradient tables obtained 
with the updated mrtrix3, versus those obtained with mri_convert, and the 
results are quite different. I attached several files, so that you could maybe 
take a look and tell me how to best interpret this:

- the bvecs from the updated mrtrix3 (mrtrix3up_bvecs)
- a screenshot from the gradient directions (FA map overlaid with V1 map) 
resulting from usage of the bvecs tables from the updated mrtrix3 
(mrtrix3up_graddirs)
- a screenshot from the path reconstruction resulting from usage of the bvecs 
tables from the updated mrtrix3 (mrtrix3up_tracts)

- the bvecs from mri_convert (mriconvert_bvecs)
- a screenshot from the gradient directions resulting from usage of the bvecs 
tables from mri_convert (mriconvert_graddirs)
- a screenshot from the path reconstruction resulting from usage of the bvecs 
tables from mri_convert (mriconvert_tracts)

Note that the TRACULA steps were done using version 6.0.

Based on these outputs, my guess is that the correct bvec table is the one 
obtained with the updated version of mrtrix and not the one obtained with 
mri_convert. But I would appreciate your opinion on that.

Thanks again for your help!

Best,

Joëlle van der Molen


________________________________
De 
:freesurfer-boun...@nmr.mgh.harvard.edu<mailto:freesurfer-boun...@nmr.mgh.harvard.edu>
 
<freesurfer-boun...@nmr.mgh.harvard.edu<mailto:freesurfer-boun...@nmr.mgh.harvard.edu>>
 de la part de Yendiki, Anastasia 
<ayend...@mgh.harvard.edu<mailto:ayend...@mgh.harvard.edu>>
Envoyé : jeudi 16 mars 2017 22:27
À : Freesurfer support list
Objet : Re: [Freesurfer] TRACULA tract reconstruction

One potential difference is that TRACULA does not need the gradient vectors to 
be flipped. It needs to get them the way they are stored in the DICOM header, 
and that's what mri_convert outputs.

Best,
a.y

________________________________
From:freesurfer-boun...@nmr.mgh.harvard.edu<mailto:freesurfer-boun...@nmr.mgh.harvard.edu>
 
[freesurfer-boun...@nmr.mgh.harvard.edu<mailto:freesurfer-boun...@nmr.mgh.harvard.edu>]
 on behalf of Antonin Skoch [a...@ikem.cz<mailto:a...@ikem.cz>]
Sent: Wednesday, March 08, 2017 10:03 AM
To: freesurfer@nmr.mgh.harvard.edu<mailto:freesurfer@nmr.mgh.harvard.edu>
Subject: Re: [Freesurfer] TRACULA tract reconstruction

Dear Joelle,

regarding the conversion to .bvecs, could you please show the example of 
difference between bvecs converted by mri_convert of FreeSurfer and mrconvert 
of mrtrix3?

Do you have most recent version of mrtrix3? There have been a quite extensive 
discussion on implementation of FSL .bvec convention in mrtrix3 in spring 2016:

http://www.mrtrix.org/2016/04/15/bug-in-fsl-bvecs-handling/

However, there have been quite a big effort to settle this issue and I am 
pretty convinced that the recent versions should have this issue resolved.

Maybe you can also post this to the mrtrix community forum, they are usually 
responding very quickly and each post gives detailed attention.

Regards,

Antonin Skoch



??Dear Tracula experts,


I am applying Tracula on a longitudinal data set, and have some questions I am
hoping you could help me with:

Firstly, upon visual inspection, several tracts appear to be quite messy, or
small (on the default freeview threshold) for most subjects. However, the -stat
step from Tracula does not necessarily flag these tracts as outliers. What is
then the best way to decide whether tracts are not correctly reconstructed and
need to be rerun separately once more? Can I rely on the flagged outliers? Or
should I rerun all the tracts that do not look ok after visual inspection? And
in the last case, for tracts that look small, are they considered as correctly
reconstructed if looking ok at lower thresholds than the default of freeview?

Second, concerning the rerunning of poorly reconstructed tracts, I was
wondering if there might be a more automated way to configure the dmrirc file
in order to adapt it for each subject/each tract to rerun (and its
corresponding control points).

Third, I was wondering if there is a recommended software for obtaining bvecs
and bvals. For the moment I used mrconvert (from MRtrix, using the
-export_grad_fsl option) and  the new mri_convert from freesurfer 6.0. As they
give different outputs, I was wondering if this may impact the reconstruction.

Lastly, would you recommend using freesurfer 5.3 or 6.0 for applying the
longitudinal stream of Tracula? Related to this, is it recommended to use the
same freesurfer version to obtain the long/base images, and to run Tracula?


Thank you for your time,


Best regards,

Joëlle van der Molen






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