Do you run an X11 virtual frame buffer - eg Xvfb?
Otherwise AFAIK R graphics and Java will complain on headless nodes.

On Sat, Jun 23, 2012 at 6:30 AM, Josh Nielsen <jniel...@hudsonalpha.com> wrote:
> Hello,
>
> I am having an issue with getting the FastQC tool to work with Galaxy on our
> server. I downloaded the FastQC files (version 0.8.0) and changed the
> directory that the wrapper script looks for the 'fastqc' executable in, but
> when we run a job with it we have been getting the following output:
>
> "Started analysis of Clip
>
> Approx 5% complete for Clip
> Approx 10% complete for Clip
> ...
> ...
> Approx 95% complete for Clip
> Approx 100% complete for Clip
>
> Analysis complete for Clip
>
> (.:9754): Gtk-WARNING **: cannot open display: "
>
> And then the job shows as failed in Galaxy. The output .dat file just has
> that same output/error message in it (though it seems to indicate it got to
> 100%). Also when I try to execute the fastqc file directly (albeit with no
> arguments) I get this:
>
> "Exception in thread "main" java.awt.HeadlessException:
> No X11 DISPLAY variable was set, but this program performed an operation
> which requires it.
>         at
> java.awt.GraphicsEnvironment.checkHeadless(GraphicsEnvironment.java:173)
> at java.awt.Window.<init>(Window.java:437)
> at java.awt.Frame.<init>(Frame.java:419)
> at java.awt.Frame.<init>(Frame.java:384)
> at javax.swing.JFrame.<init>(JFrame.java:174)
> at
> uk.ac.bbsrc.babraham.FastQC.FastQCApplication.<init>(FastQCApplication.java:271)
> at
> uk.ac.bbsrc.babraham.FastQC.FastQCApplication.main(FastQCApplication.java:102)"
>
> Both errors seem to have something to do with the graphical GUI component of
> FastQC (which I have seen some screenshots for on the FastQC webpage). If
> this application is GUI-driven how did the online PSU Galaxy get it to work
> with their wrapper script when the tools are run in a command-line
> environment with no X11 or Gtk? Essentially I'm just wondering what steps
> I'm missing here to getting this to work with our Galaxy mirror, other than
> just dropping the executable in place? Any suggestions?
>
> Thanks,
> Josh
>
>
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-- 
Ross Lazarus MBBS MPH;
Associate Professor, Harvard Medical School;
Head, Medical Bioinformatics, BakerIDI; Tel: +61 385321444;

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