This is due to a mismatch is chromosome names between your hg19.len file (UCSC 
naming) and the VCF (Ensembl naming). This should be handled better by Galaxy, 
and I’ve created a card for it:

https://trello.com/c/bjmNesZj

In the meantime, I used this command to convert chromosome names for this VCF:

sed -E 's/^[0-9XY]+/chr&/‘ input.vcf | sed -E 's/^MT/chrM/'

You may need to massage it depending on your platform and sed version.

Best,
J.

--
Jeremy Goecks
Assistant Professor of Computational Biology
George Washington University



On Apr 20, 2014, at 11:27 AM, Saket Choudhary <sake...@gmail.com> wrote:

> I am trying to visualise VCF exported from:
> ftp://ngs.sanger.ac.uk/production/cosmic/CosmicCodingMuts_v68.vcf.gz
> 
> However I get the following error: (Both on Galaxy Main and Local 
> installation)
> 
> Input error: Chromosome 1 found in your input file but not in your genome 
> file.
> needLargeMem: trying to allocate 0 bytes (limit: 100000000000)
> 
> 
> The input file seems to be conforming to VCF4.1 format.  Is this an
> issue with trackster instead?
> 
> 
> Saket
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