Hi Peter,

Thank a lot for this information, I didn't found this option for the new version of the Data Libraries.
This could explain why there is this conversion.
I tested it with an automatic format detection and when I set explicitly the format but in the two cases a tab was added.

Fabien

Le 18/03/2016 17:04, Peter Cock a écrit :
Hi Fabien,

There is a Galaxy upload option to turn spaces into tabs, useful for
tables of data. Is that being ticked by mistake? That would explain
this problem.

Also I would check if the FASTQ file format is being detected
automatically, or set explicitly (e.g. fastqsanger)? Doing that ought
to prevent any changes.

Peter

On Fri, Mar 18, 2016 at 3:26 PM, Fabien Mareuil <fmare...@pasteur.fr> wrote:
Hi,

We got a problem with the new version of Data libraries. When we upload a
fastq file from a User Directory, a tabulation character is added in the
header in replacement of a space character.

from @HWI-D00395:188:HYGNFADXX:2:1108:1315:1965 1:N:0:ATGAGC to
@HWI-D00395:188:HYGNFADXX:2:1108:1315:1965      1:N:0:ATGAGC


This behavior causes an error with cutadapt 1.6 (maybe with other tools)
because it doesn't work with tab character. With the direct upload tool or
the deprecated version of Data Libraries no tab character is added. Do you
have some idea to correct this problem? Thanks a lot Best regards --
Fabien Mareuil | Centre d'Informatique pour la Biologie
fabien.mare...@pasteur.fr | Institut Pasteur 25,28 rue du Docteur Roux 75015
Paris, France

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