Hi Deepak,

how are you running this Container?
Can you check how many memory you have inside the container?

Cheers,
Bjoern

On 11.08.2017 15:35, Deepak Tanwar wrote:
> Hello,
> 
> I am trying to build Bowtie2 index for hg19.
> I a using Docker image of Galaxy.
> 
> Computer I am using have 12 cores and 64 GB of RAM.
> 
> Error I am getting is:
> 
> Fatal error: Exit code 1 ()
> Building a SMALL index
> Could not allocate space for a joined string of 2897310462 elements.
> Switching to a packed string representation.
> Building a SMALL index
> Could not find approrpiate bmax/dcv settings for building this index.
> Already tried a packed string representation.
> Please try indexing this reference on a computer with more memory.
> Error: Encountered internal Bowtie 2 exception (#1)
> Command: bowtie2-build --wrapper basic-0
> /export/galaxy-central/database/job_working_directory/000/113/dataset_80_files/hg19
> fa.fa hg19 fa 
> Deleting "hg19 fa.3.bt2" file written during aborted indexing attempt.
> Deleting "hg19 fa.4.bt2" file written during aborted indexing attempt.
> Deleting "hg19 fa.1.bt2" file written during aborted indexing attempt.
> Deleting "hg19 fa.2.bt2" file written during aborted indexing attempt.
> Deleting "hg19 fa.3.bt2" file written during aborted indexing attempt.
> Deleting "hg19 fa.4.bt2" file written during aborted indexing attempt.
> Deleting "hg19 fa.1.bt2" file written during aborted indexing attempt.
> Deleting "hg19 fa.2.bt2" file written during aborted indexing attempt.
> Error building index. 
> 
> Please help!
> 
> Thank you,
> Deepak
> 
> 
> 
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