Hello, First of all thank you for all of your hard work developing such an informative and intuitive tool. I have been successful in running the LEfSE analyses for some of my data, but unfortunately, for the majority I get an error message when it comes to the step where the Effect size is calculated. The error message is copied below:
/galaxy_venv/local/lib/python2.7/site-packages/rpy2/rinterface/__init__.py:185: RRuntimeWarning: Error in (function (file = "", n = NULL, text = NULL, prompt = "?", keep.source = getOption("keep.source"), : <text>:1:47: unexpected numeric constant 1: effect.size <- abs(mean(LD[sub_d[,"class"]==""1 ^ warnings.warn(x, RRuntimeWarning) Traceback (most recent call last): File "/shed_tools/testtoolshed.g2.bx.psu.edu/repos/george-weingart/lefse/a6284ef17bf3/lefse/run_lefse.py", line 89, in <module> if params['rank_tec'] == 'lda': lda_res,lda_res_th = test_lda_r(cls,feats,class_sl,params['n_boots'],params['f_boots'],params['lda_abs_th'],0.0000000001,params['nlogs']) File "/export/shed_tools/testtoolshed.g2.bx.psu.edu/repos/george-weingart/lefse/a6284ef17bf3/lefse/lefse.py", line 199, in test_lda_r robjects.r('effect.size <- abs(mean(LD[sub_d[,"class"]=="'+p[0]+'"]) - mean(LD[sub_d[,"class"]=="'+p[1]+'"]))') File "/galaxy_venv/local/lib/python2.7/site-packages/rpy2/robjects/__init__.py", line 358, in __call__ p = _rparse(text=StrSexpVector((string,))) rpy2.rinterface.RRuntimeError: Error in (function (file = "", n = NULL, text = NULL, prompt = "?", keep.source = getOption("keep.source"), : <text>:1:47: unexpected numeric constant 1: effect.size <- abs(mean(LD[sub_d[,"class"]==""1 ^ For your reference, I am using this tool to analyze human gut microbiome data. Also, I cross-posted to the forum (link: https://help.galaxyproject.org/t/lda-effect-size-error/2840) Any guidance would be greatly appreciated. all the best, Caroline -- Caroline M. Kelsey Developmental Psychology Ph.D. Candidate UVA Babylab: http://uvababylab.org/ The University of Virginia ___________________________________________________________ Please keep all replies on the list by using "reply all" in your mail client. To manage your subscriptions to this and other Galaxy lists, please use the interface at: %(web_page_url)s To search Galaxy mailing lists use the unified search at: http://galaxyproject.org/search/