Hello Christopher,

This wiki explains the exact steps for how to set up reference genomes for NGS tools, including BWA:
http://wiki.g2.bx.psu.edu/Admin/NGS%20Local%20Setup

Please let us know if you have any questions. For local instance questions, searching prior answers (Nabble) or posting to galaxy-...@bx.psu.edu is recommended.
http://galaxyproject.org/wiki/Mailing%20Lists

Take care,

Jen
Galaxy team

On 11/9/11 11:02 AM, Christopher Callaway wrote:
Hello,

I am still having problems understanding how to install mm9 mouse ref
genome into our own install of Galaxy. When I try to map with BWA for
Illumina the drop down for select ref genome is not populated with
anything. How do you grab the mm9 ref file and how do you convert it so
that Galaxy local “sees” it? How do you build the index and how do you
prepare the loc file? Our install does not contain a folder
tool-data/shared/ucsc/builds.txt.

Sorry but I am not a programmer, just a bench scientist.

Thanks in advance,

Christopher W. Callaway

University of Utah

Dept. of Pediatrics

Division of Neonatology

417 Wakara Way

#2222

Salt Lake City, UT 84108



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