Baohua and Jane,

As David noted, there is a Trinity wrapper for Galaxy, it works, and Trinity is 
great. 

However, Trinity is not enabled/installed on our public server (main.g2) or on 
Galaxy cloud instances (Amazon) right now. You'll need a little programming 
expertise to set up Trinity locally or on the cloud. Also, the Galaxy's team 
support for Trinity is very minimal right now as we haven't done much testing 
with it yet.

Good luck,
J.

On Dec 21, 2011, at 4:01 PM, David Matthews wrote:

> Hi Jane,
> 
> I have used Trinity on a local installation here at Bristol University. The 
> main reason its not on Galaxy main is because its very very memory intensive 
> (we run it on nodes with 256GB RAM). So you really need access to a big 
> machine to run it. Having said all that the output is astoundingly good so 
> it's worth the time and effort to get a run going if you can.
> 
> Cheers
> David
> 
> 
> 
> On 21 Dec 2011, at 13:36, Jane Song wrote:
> 
>> Dear Galaxy Expert,
>> 
>> I would like to use Galaxy to de-novo assembly single-end read illumina data 
>> (140bp) for plant transcriptomes (without reference).  I remember early 
>> emails mention trinity in Galaxy. But I could not see at Galaxy web 
>> http://main.g2.bx.psu.edu/root
>> . Maybe it is installed in Amarzon EC2? Other suggestions in de-novo 
>> assembly plant transcriptomes without reference.
>> 
>> Many thanks and look forward to hearing back from you,
>> Jane
>> 
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> ___________________________________________________________
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___________________________________________________________
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