Hello Irene,

This wiki has the formatting details, including a screencast for genome prep, and many tips for correcting format problems that use Galaxy's tools whenever possible:

http://wiki.g2.bx.psu.edu/Learn/CustomGenomes#Screencasts_.26_Tutorials
watch "Custom Genome Prep"

http://wiki.g2.bx.psu.edu/Learn/CustomGenomes#Troubleshooting

That said, it many simply be that the "datatype" is not actually set to be "fasta" for the dataset? Do this by clicking on the pencil icon to reach the "Edit Attributes" form, set this manually, and save.

Hopefully this helps,

Jen
Galaxy team

On 8/10/12 3:39 AM, i b wrote:
Dear all,
I managed to upload to Galaxy a genome of interest in .fasta format
from NCIB website.

However, Galaxy does not recognize it as input to run Tophat...

Wht format has to be to be used as referece genome for tophat?and how
can i convert it?

Any suggestion?

thanks,
ib
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