Hello Miro,
It sounds like perhaps the datatype is not being assigned correctly,
which may mean that they quality scores are not scaled properly. To
double check both, see the instructions in our wiki here:
http://wiki.galaxyproject.org/Support#Dataset_special_cases
If you still need help after this, please write back to share a history,
Best,
Jen
Galaxy team
On 11/29/13 5:49 AM, miroslav.sotak wrote:
To whom it may concern
I do have a problem with tophat. I can easily put fastq data to
"history" and according to RNA-seq Analysis Exercise provided by
Jeremy. We checked the type of Ascii ofset for the quality estimation.
I tried even "quality data converter" set to 33 (we do have data of
this ASCII offset from 2 different sources) but "tophat for Illumina"
simply can not read the data before and even after quality format
converter. We do not have any idea what is going on. I am logged in
Galaxy with current email, can you check my data and is there any
converter for quality offset?
Sincerely
Miro Sotak
___________________________________________________________
The Galaxy User list should be used for the discussion of
Galaxy analysis and other features on the public server
at usegalaxy.org. Please keep all replies on the list by
using "reply all" in your mail client. For discussion of
local Galaxy instances and the Galaxy source code, please
use the Galaxy Development list:
http://lists.bx.psu.edu/listinfo/galaxy-dev
To manage your subscriptions to this and other Galaxy lists,
please use the interface at:
http://lists.bx.psu.edu/
To search Galaxy mailing lists use the unified search at:
http://galaxyproject.org/search/mailinglists/
--
Jennifer Hillman-Jackson
http://galaxyproject.org
___________________________________________________________
The Galaxy User list should be used for the discussion of
Galaxy analysis and other features on the public server
at usegalaxy.org. Please keep all replies on the list by
using "reply all" in your mail client. For discussion of
local Galaxy instances and the Galaxy source code, please
use the Galaxy Development list:
http://lists.bx.psu.edu/listinfo/galaxy-dev
To manage your subscriptions to this and other Galaxy lists,
please use the interface at:
http://lists.bx.psu.edu/
To search Galaxy mailing lists use the unified search at:
http://galaxyproject.org/search/mailinglists/
