Good Evening:

With these large bed files, you are going to have trouble
with any of the formats unless you have a very large memory
machine.  Large as in 250 Gb of memory or more.

I would suggest breaking up your bed file per chromosome.
Load the parts with table names:
chr1_trackName
chr22_trackName
... etc ...

These tracks will function as a single track by the name of "trackName"
Use "trackName" in your trackDb entry.  This is called a 'split'
table and the genome browser knows how to access each set
of data per chromosome.  Take a look at example table names
in the hg18 database.  Many tracks are loaded like this.

Even if you tried to encode this file with bedToBigBed you would
still need a very large machine.  Probably even larger than
the hgLoadBed command requires.

--Hiram

Jennifer Jackson wrote:
> Hello,
> 
> It seems like you are using your own mirror and trying to upload there as a 
> native track? From a first look, you are out of disc space.
> 
> Double check that you have space, then perhaps try an alternate format 
> designed for large datasets, such as bigBed or bigWig.
> 
> And finally, breaking the data up per-chromosome is always an option. We do 
> this for many tracks internally.
> 
> Hiram may have more to add later on,
> 
> Thanks,
> Jennifer
> 
> ps. Just to double check, your mySQL version is still 4, but we are on 5. Be 
> certain that you are using a consistent source code tree for all (data and 
> software). Some changes were made to several utilities when the switch to v5 
> was made. See the genome-mirror mailing list archives for exact changes. 
> http://genome.ucsc.edu/contacts.html#mirror
> 
> 
> ------------------------------------------------ 
> Jennifer Jackson 
> UCSC Genome Bioinformatics Group 
> 
> ----- "WANG Wuqi" <[email protected]> wrote:
> 
>> From: "WANG Wuqi" <[email protected]>
>> To: "[email protected]" <[email protected]>
>> Cc: "Jennifer Jackson" <[email protected]>, [email protected], "WANG Wuqi" 
>> <[email protected]>
>> Sent: Tuesday, November 10, 2009 7:39:38 PM GMT -08:00 US/Canada Pacific
>> Subject: UCSC  data loading error - needMem: Out of memory
>>
>> Hi Hiram,
>>
>> When we upload  BED data format with 12 columns to encounter below
>> error:
>> ................
>> $ hgLoadBed hg18 RHM005_final_gff_noFILT RHM005_final_gff_noFILT.bed
>> Reading RHM005_final_gff_noFILT.bed
>> needMem: Out of memory - request size 89 bytes, errno: 12
>> ................
>>
>> The size of this data file is about 3GB. When we take part of data,
>> for example, 1000 rows
>> to do custom track or load, both are ok.
>>
>> Our server:
>> Linux ucsc.fugu-sg.org 2.6.9-67.0.22.ELsmp #1 SMP Wed Jul 23 17:30:51
>> EDT 2008 i686 i686 i386 GNU/Linux
>>
>> MySQL: Ver 14.7 Distrib 4.1.22, for pc-linux-gnu (i686) using readline
>> 4.3
>>
>>
>> Thanks & regards
>>
>> Wuqi
> 
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