Hello,
I compared the mRNA sequence block for the TTN gene obtained from (1): http://genome.ucsc.edu/cgi-bin/hgc?hgsid=147684681 <http://genome.ucsc.edu/cgi-bin/hgc?hgsid=147684681&g=htcCdnaAli&i=NM_133378 &c=chr2&l=179098963&r=179380395&o=179098963&aliTrack=refSeqAli&table=refGene > &g=htcCdnaAli&i=NM_133378&c=chr2&l=179098963&r=179380395&o=179098963&aliTrac k=refSeqAli&table=refGene) with the concatenation of exon coding sequences obtained from (2): http://genome.ucsc.edu/cgi-bin/hgc?hgsid=147684681 <http://genome.ucsc.edu/cgi-bin/hgc?hgsid=147684681&g=htcGeneInGenome&i=NM_1 33378&c=chr2&l=179098963&r=179380395&o=refGene&table=refGene> &g=htcGeneInGenome&i=NM_133378&c=chr2&l=179098963&r=179380395&o=refGene&tabl e=refGene and found many differences which, in part, appear to be the result of including pieces of intronic sequence with exons in the latter step (2). I used the ATG in block two (from the mRNA block) and stripped off all sequence before this start codon in both sequence sets before the comparison. I wonder if the process that extracts the exons (step 2) might have a problem with this large gene. Regards, Bill Dickinson _______________________________________________ Genome maillist - [email protected] https://lists.soe.ucsc.edu/mailman/listinfo/genome
