Hello Angie,

In one of your previous emails, you described the automated way to "Get
DNA." In this description, step six stated:

6. Next, the sequences are passed to generateAlignment.  Search
backwards in the source to see the definition of generateAlignment.
Note that it calls axtScoreSchemeSimpleDna to get a scoring matrix and
gap penalties, and then passes the sequences and scoring scheme to
axtAffine.  See kent/src/lib/axt.c and kent/src/lib/axtAffine.c .  The
realignment is printed out block by block using printOffsetAndBoldAxt.

I would like to know how the program generateAlignment is called/used. Can
it be called in a command line, or perhaps with Blat? Where can I find
information on using this program?



Kyle Tretina
Wheaton College
Junior
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