Hi Ruth, You can always reverse complement the strand by pressing the reverse button at the top of the track groups, which will also reverse complement the conservation track. You can also complement the strand by pressing the arrow button (--->) at the top of the base position track (when you are zoomed in to at least the base level). However it is important to realize that due to the strict base pairing of DNA, the conservation of one strand is equivalent to the conservation of the other strand. In other words, you do not need to reverse complement your strand in order to see the conservation for a base.
I hope this information is helpful. Please feel free to contact the mail list again if you require further assistance. Best, Mary ------------------ Mary Goldman UCSC Bioinformatics Group On 4/29/10 8:30 AM, Ruth Bennett wrote: > Hi, > I am looking at a region on human chromosome 11 around the loci SLC22A18 > & SLC22A18AS. > There is a potential ORF for the locus SLC22A18AS which codes for 40aa > (MENCMKRQRSAVCPHPSPPGLQLVGTGFCGCRGLPQILPH*) and I wanted to check the > conservation of the first methionine. > How can I change the conservation track to show me the conservation of > the other strand as it currently only shows the conservation of SLC22A18 > in this area (VLAATELTCLFMQFSIVP)? > I would be grateful for any help. > Kind regards. > Ruth Bennett > > > _______________________________________________ Genome maillist - [email protected] https://lists.soe.ucsc.edu/mailman/listinfo/genome
