Hello Yongsheng, There is no limit to the size of the files, but as you have noticed, larger files to tend to load very slowly. One alternative is to convert your files to the bigBed format. You can read about here:
http://genome.ucsc.edu/FAQ/FAQformat.html#format1.5 http://genome.ucsc.edu/goldenPath/help/bigBed.html You could also try breaking up your file into a group of smaller bed files. I hope this answers your question. Please feel welcome to contact us again. Antonio Coelho UCSC Genome Bioinformatics Group Yongsheng Bai wrote: > Hi, > > Is there any file size limit for loading a bed file into UCSC's custom > track? I am loading a ~200MB file, it takes forever... > > Thanks. > YB > > -----Original Message----- > From: Jennifer Jackson [mailto:[email protected]] > Sent: Monday, May 03, 2010 2:17 PM > To: [email protected] > Subject: Re: [Genome] download hg18 genomic sequences > > Hello, > > The track GC Percent may be helpful. It is based on the > reference genome. > > quote from GC Percent track description page: Description > > The GC percent track shows the percentage of G (guanine) > and C (cytosine) bases in 5-base windows. High GC content > is typically associated with gene-rich areas. > > You could BLAT your sequence against the reference genome > and view the region or note the coordinates, then export > data from this track using the Table browser. > > Or for batch use, if you know the coordinates, upload them > into the region filter in the Table browser and export data > from the GC Percent track or use the file from downloads (below). > > To this in batch on the command line to generate GC content for your own > sequences, use the utility percentage from the kent source tree. It > would take input data that you format. Please ask if you need help to > convert your files to the required formats. > > Table browser help: > http://genome.ucsc.edu/goldenPath/help/hgTablesHelp.html > > Link to description of utilities (scroll to hgGcPercent): > http://hgwdev.cse.ucsc.edu/~larrym/utilities.html > > Download source: > http://genome.ucsc.edu/FAQ/FAQdownloads.html#download27 > > File formats: > http://genome.ucsc.edu/FAQ/FAQformat.html > > Complete GC content file for latest human reference genome: > http://hgdownload.cse.ucsc.edu/goldenPath/hg19/gc5Base/ > > Best wishes with your project, > Jennifer > > ps: It would be best if you would send new questions directly to the > mailing list at [email protected]. This helps us to get you the > quickest reply. > > --------------------------------- > Jennifer Jackson > UCSC Genome Informatics Group > http://genome.ucsc.edu/ > > On 5/3/10 10:30 AM, Yongsheng Bai wrote: > >> Hi Jennifer, >> >> Does the UCSC allow or have the functionality to view/calculate GC >> > contents > >> given an input sequence? >> >> Thanks, >> YB >> >> > > > > _______________________________________________ > Genome maillist - [email protected] > https://lists.soe.ucsc.edu/mailman/listinfo/genome > _______________________________________________ Genome maillist - [email protected] https://lists.soe.ucsc.edu/mailman/listinfo/genome
