Hi Marg, Assemblies, such as hg18 vs. hg19, can have differences like the ones you have described. Is it possible that you developed your primers on a different assembly than you viewed the next day? If this isn't the case, please contact the mailing list again.
Vanessa Kirkup Swing UCSC Genome Bioinformatics Group ----- Original Message ----- From: "Margaret C Skokan" <[email protected]> To: [email protected] Sent: Thursday, June 3, 2010 2:34:08 PM GMT -08:00 US/Canada Pacific Subject: [Genome] data shift? To whom it may concern It looks as though there has been a data shift of about 200kb overnight. Is there something wrong with the browser? I selected eight sets of clones and developed primers yesterday and when I returned to review everything today I noticed a dramatic difference in the start and end points of my clones. Thanks Marg Margaret C Skokan, PRA Dr. Marileila Garcia's Laboratory Department of Medicine/Cancer Center University of Colorado Health Sciences Center Phone: (303) 724-3148 Fax: (303) 724-3889 Mailing Address: Campus Box 8117 PO Box 6511 Aurora, CO 80045 Street Address: RC-1 South Building, Room L18-8401 12801 East 17th Avenue Aurora, CO 80045 _______________________________________________ Genome maillist - [email protected] https://lists.soe.ucsc.edu/mailman/listinfo/genome _______________________________________________ Genome maillist - [email protected] https://lists.soe.ucsc.edu/mailman/listinfo/genome
