Hello - Double checking the format is the first step http://genome.ucsc.edu/goldenPath/help/wiggle.html
Data Values Wiggle track data values can be integer or real, positive or negative values. Chromosome positions are specified as 1-relative. For a chromosome of length N, the first position is 1 and the last position is N. Only positions specified have data. Positions not specified do not have data and will not be graphed. All positions specified in the input data must be in numerical order. It appears that you have a some bases covered at least twice in the input wigs. This needs to be resolved. Compare the declaration lines in the wig files to find the problem areas. Thanks, jen --------------------------------- Jennifer Jackson UCSC Genome Informatics Group http://genome.ucsc.edu/ On 6/9/10 8:01 AM, Chengdong Zhang wrote: > Hi there, > > > > I am now trying to get one BigWig for my Wig Track files. > > > > I have chr1- chrY, and I combine them into one wig file, > > > > Then use ./wigToBigWig ge6-d0-k27me3-sorted-unique_sample.wig > rn4.chrom.sizes ge6-d0-k27me3-sorted.unique.bw > > > > But it keeps popping out: > > > > There's more than one value for chr7 base 74237123 (in coordinates that > start with 1). > > > > . > > . > > . > > There's more than one value for chr4 base 23546688 (in coordinates that > start with 1). > > > > > > Any suggestion? > > > > Thank you very much for your reply. > > > > Have a great day! > > > > > > Chengdong > > _______________________________________________ > Genome maillist - [email protected] > https://lists.soe.ucsc.edu/mailman/listinfo/genome _______________________________________________ Genome maillist - [email protected] https://lists.soe.ucsc.edu/mailman/listinfo/genome
