Hi Vivian, Thank you for your offer. If you would like, we could link to your data on your server from our custom tracks page: http://genome.ucsc.edu/goldenPath/customTracks/custTracks.html. Please let us know if this is something that you are interested in arranging.
Best, Mary --------------------- Mary Goldman UCSC Bioinformatics Group On 6/21/10 8:15 AM, [email protected] wrote: > I am writing to inquire if you are interested in having ChIP-chip (mouse > promoter array) data from mouse testis for the transcription factor DMRT1 > to distribute as part of you browser. We have a PNAS publication in press > on this and have tracks from three independent IP experiments. We will be > including the tracks on a interactive web site that will be freely > available to readers but thought you might want to host the tracks as well. > The abstract of the paper is copied below. > We need a quick answer as we need to return the proofs today and need > to know whether to say the tracks will be available on your genome browser. > Our web site will be up and running later today and I can provide you > with a URL if you are interested. > Thank you for your consideration of this matter. > Vivian Bardwell > > > > Genome wide analysis of DNA binding and transcriptional regulation by the > mammalian Doublesex homolog DMRT1 in the juvenile testis > Mark M. Murphy1, Aaron L. Sarver2, Daren Rice3, Katerina Hatzi4, Kenny Ye5, > Ari Melnick4, Leslie L. Heckert3, David Zarkower1, Vivian J. Bardwell1* > ABSTRACT > Doublesex- and MAB-3-related transcription factors (DM domain > proteins) are widely conserved in metazoan sex determination and sexual > differentiation. One of these proteins, DMRT1, plays diverse and essential > roles in development of the vertebrate testis. In mammals DMRT1 is > expressed and required in both germ cells and their supporting Sertoli > cells. Despite its critical role in testicular development little is known > of how DMRT1 functions as a transcription factor or what genes it binds and > regulates. Here we combine chromatin immunoprecipitation methods with > conditional gene targeting and mRNA expression analysis. This identified > almost 1400 promoter-proximal regions bound by DMRT1 in the juvenile mouse > testis and determined how expression of the associated mRNAs is affected > when Dmrt1 is selectively mutated in germ cells or Sertoli cells. These > analyses revealed that DMRT1 is a bifunctional transcriptional regulator, > activating some genes and repressing others. ChIP analysis using > conditional mutant testes showed that DNA binding and transcriptional > regulation of individual target genes can differ between germ cells and > Sertoli cells. Genes bound by DMRT1 in vivo were enriched for a motif > closely resembling the sequence DMRT1 prefers in vitro. Differential > response of genes to loss of DMRT1 corresponded to differences in the > enriched motif, suggesting that other trans-acting factors may modulate > DMRT1 activity. DMRT1 bound its own promoter and those of six other Dmrt > genes, indicating auto- and cross regulation of these genes. Many of the > DMRT1 target genes identified here are known to be important for a variety > of functions in testicular development; the others are candidates for > further investigation. > > _______________________________________________ > Genome maillist - [email protected] > https://lists.soe.ucsc.edu/mailman/listinfo/genome > _______________________________________________ Genome maillist - [email protected] https://lists.soe.ucsc.edu/mailman/listinfo/genome
