Dear list,
I am trying to visualize alignment of my Illumina reads in the Genome Browser.
The result I am trying to get is a track where I can see the reads piling up,
as if they were peaks of alignment, as is shown in many articles and
presentations. To do so, as I want to load alignment against the whole mouse
genome, I transformed my Illumina _sorted.txt to BED format and this BED format
to BigBed format so that transfering data to the Genome Browser was faster. But
I am getting just plain rectangular boxes at the given positions of alignment
and not a nice shaped peak that would allow me to rougly compare peak heights
visually.
Where am I going wrong?
Thanks for your help
Dave
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