Hello, I want to consult on how to understand the "Refgene.txt" file. For example " 1654 NM_031497 chr5 + 140180782 140183255 140180782 140183255 1 140180782, 140183255, 0 PCDHA3 cmpl incmpl 0, 971 NR_024227 chr19 - 50595745 50595866 50595866 50595866 1 50595745, 50595866, 0 SNAR-A6 unk unk -1, " If I would like to locate the Tss of the first gene, so I go through the sequence of chr5, starting from the first base which count as "0", until the base whose count equal to "cdsStart". Is this correct? Besides, how can I locate the second gene then? Do I search from the beginning of the reverse compliment of chr19 sequence? I mean is the first base on 5' direction of the other strand of chr19 is viewed as the first base now and count as "0"?
Best, Yunfei Li -------------------------------------------------------------------------------------- Research Assistant Department of Statistics & School of Molecular Biosciences Biotechnology Life Sciences Building 427 Washington State University Pullman, WA 99164-7520 Phone: 509-339-5096 http://www.wsu.edu/~ye_lab/people.html _______________________________________________ Genome maillist - [email protected] https://lists.soe.ucsc.edu/mailman/listinfo/genome
