Hello, Recently, I allign my human sequence with UCSC, it shows the sequence is the same with forward strand. However, the sequence is the oppsite with the cDNA orentation (NM_004898). It seems to me that one orentation could be wrong. Could you please help me? For you convenience, I send you the sequence as attached. Thanks.
TCTAGACATA TACATATTTT TACATCATCT GTACATAGAG AATTTGAACC 50 CACACAACAT TCTGAAAGTA ATTACTGTTC CATCATTATC TGAAAAGGGA 100 GAGGGGTTGG GGGAGGGGGT CGCTACCAAG TCTACTGGAC TGTCTTCATG 150 GCATCACTGT AAGCAAACCC CTGACATGGC AGTGGTATGT CCTCTGTAAC 200 ACTTGATAAG AGGCACAGAA CCACTAAAAG TTACTAACAT CATTAGTGCC 250 TTTCTGATTC CCTGGAGGTC ATTTCATAGC TGAGCTTCTT AATATTTGGC 300 AATATATTCT TTTTCCATCA AAAAATATCC AGGCACCTAA AACACTGTCA 350 GAACTGGCTg TGCCCCTATG ATCACCTCCT GCTGGCTGGT ATTTAATGTA 400 CATCTGTAGC ACTAGGCAGC ATTTTCTCTG CCAACTAATT CCAGGAACTA 450 GAACACTCAA TACTGCATCT CATGAAACTG CTGGAACTTT CCCTCCTTTC 500 CTCAGGTCAT CTGAGTAACT CTTAATGGGC CATCCCCTTC CTCCCTTGAT 550 GTCAAGAGAG GAAGCACGaG TGCTACTGTG GTTGAAC
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