Hi, 

I'm currently setting up a local mirror of a genome browser in order to 
visualize NGS data internally. The main type of data is human exome data, where 
we have now ≈50 exomes sequenced. 

I have been following the progress of BAM support in UCSC GB and it looks very 
promising. One thing that I however miss is to be able to display density plots 
directly from BAM files, either as 1) a plot with coverage along the y-axis, or 
2) as a density with a color indicating coverage. I attached a screendump where 
my suggestion 1) would be similar to the Enhancer H3K4 track and the suggestion 
2) would be similar to the CRG Align tracks (ie scale from white to black if 
coverage is 0-50, otherwise black). 

It would also be awesome if it would be possible to pick custom colors in the 
settings for each track, instead of being locked to black and gray. 

If I understand correctly, samtools-c is able to create pileups, which could 
then be used to create a bedgraph on the fly. I really don't want to create a 
separate set of bedgraph files for each sample. 

Thanks a lot, 

Daniel Klevebring






--
 
Contact information:
 
Daniel Klevebring, Ph. D.
Department of Medical Epidemiology and Biostatistics
Karolinska Institute
Nobels Väg 12A, P. O. Box 281
SE-17 177 Solna
Sweden
 
Phone: +46 704 71 65 91 (Mobile)

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