Hello Jian,

In short, the start coordinate should be reduced by -1.

To see a full description of the 0-based, fully closed coordinate system 
in use by UCSC for most of our file formats, including BED, please see 
this discussion:
http://genomewiki.cse.ucsc.edu/index.php/Coordinate_Transforms


Please feel free to contact the mailing list support team again if you 
would like more assistance.

Warm regards,

Jen
UCSC Genome Browser Support

On 9/11/10 10:57 PM, Yuan Jian wrote:
> Hello,
>
> when I put 1-length block in bed file:
> chr1    557204  557204  FC 2-5  0       +       557204  557204  0,0,45  1     
>   1       0
>
> I got an error below:
> line 1 of xxx.sorted.bed: start and end coordinates the same
> They need to be at least one apart
>
> what is the correct way to describe 1-length block?
>
> thanks
> Jian
>
>
>
>
>
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