Hello Xiake, Some easier ways to retrieve the genomic sequence (for any species):
1) Use the "Get DNA" tool in that species home browser. First adjust the browser window to the position, then submit and download. 2) Use the Table Browser. Either enter the coordinates in directly for a single location (using track Assembly, table gold) or create a custom track BED file to extract in batch (create/load custom track then open in TB). Select output as sequence for both methods. 3) Download the complete genomes from Downloads and extract using unix tools. Some help links: http://genome.ucsc.edu/FAQ/FAQdownloads#download1 http://genome.ucsc.edu/FAQ/FAQdownloads#download32 http://genome.ucsc.edu/goldenPath/help/hgTracksHelp.html#TableBrowser http://genome.ucsc.edu/FAQ/FAQcustom http://genome.ucsc.edu/goldenPath/help/hgTracksHelp.html#CustomTracks Since you already know the coordinates, this process should be fairly straightforward, but please let us know if you need more help or if we misunderstood your question. Thanks! Jennifer Jackson UCSC Genome Bioinforamtics Group ----- Original Message ----- From: "irix fun" <[email protected]> To: [email protected] Sent: Monday, July 20, 2009 8:09:06 AM GMT -08:00 US/Canada Pacific Subject: [Genome] How to get the completed multiple-species alignment? Hello, I am trying to get the multiple species alignment from "15-way insect alignment" locally. After parsing the result from lots of "MAF" file, I found that some indels exist in another species (not the reference species) but it's hard to get the sequence directly from "MAF" file. Does anybody get some ideas to solve this? Thanks very much. Xiake _______________________________________________ Genome maillist - [email protected] https://lists.soe.ucsc.edu/mailman/listinfo/genome _______________________________________________ Genome maillist - [email protected] https://lists.soe.ucsc.edu/mailman/listinfo/genome
