Hi Hiram,

Thanks for pointing out this wiki. Since the MAF format description on the
USCS browser help-page does not make this interpretation explicit (see
http://genome.ucsc.edu/FAQ/FAQformat.html#format5), may I suggest to put a
link to this wiki there? I think it would benefit lots of folks out there.

Thanks,
Jaaved

-----Original Message-----
From: Hiram Clawson [mailto:[email protected]] 
Sent: Monday, January 31, 2011 12:11 PM
To: Jaaved Mohammed
Cc: [email protected]
Subject: Re: [Genome] Insect MAF file coordinate-sequence disagreement

See also:

http://genomewiki.ucsc.edu/index.php/Coordinate_Transforms

Brian Raney wrote:
> We realize that these coordinate systems can be confusing, but using
> these systems internally makes it easier to avoid different code for
> alignments on the different strands.
> 
> On Thu, Jan 27, 2011 at 2:15 PM, Jaaved Mohammed <[email protected]>
wrote:
>> Hello,
>>
>> I am seeing numerous discrepancy between the sequences and coordinates in
>> the insect 15-way multiple-alignment files
>> (http://hgdownload.cse.ucsc.edu/goldenPath/dm3/multiz15way/) primarily
for
>> D. ananassae and D. virilus. Hopefully I can explain this as concisely as
>> possible, however, let me know how I can clarify:

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