Hi, I am getting a very strange results when I compare the sequence of chr13 (mouse mm8/NCBI36 release Feb2006) obtained as ftp as a chr13.fa.gz(retrived from link http://hgdownload.cse.ucsc.edu/goldenPath/mm8/chromosomes/chr13.fa.gz ) (call this a seqChr13_A.fa) and the DNA sequence of same chr13 obtained via link http://genome.ucsc.edu/cgi-bin/hgc?hgsid=189316231&o=0&g=getDna&i=mixed&c=chr13&l=0&r=120614378&db=mm8&hgsid=189316231( http://genome.ucsc.edu/cgi-bin/hgc?hgsid=189316231&g=htcGetDna2&table=&i=mixed&o=0&l=0&r=120614378&getDnaPos=chr13%3A1-120%2C614%2C378&db=mm8&hgSeq.cdsExon=1&hgSeq.padding5=0&hgSeq.padding3=0&hgSeq.casing=upper&hgSeq.maskRepeats=on&boolshad.hgSeq.maskRepeats=0&hgSeq.repMasking=lower&boolshad.hgSeq.revComp=0&submit=get+DNA<[email protected]>) (call this a seqChr13_B.fa )
What is strange is the FTP obtained Chr13 seq (seqChr13_A.fa) has 12614378 bases which is correct as per the statistics of http://genome.ucsc.edu/cgi-bin/hgTracks?hgsid=189336755&chromInfoPage= However, when I check the same chr sequence obtained via browser (seqChr13_B.fa ), it shows that the chr has only 54821374 bases. So what happened to almost ~65MB sequences? Is this a known bug? Or Is this something to do with internet browser not capable of loading 120MB file and only loading the 54MB file in the browser? But then when I check the end of the two files, the last 50 line sequence match correctly, means the end of file was downloaded correctly via broswer. (Not to mentioned, I checked various browsers on winodws/linux (both 32 and 64 bit) with Internet explorer/firefox/google chrome and I get the same file back as (seqChr13_B.fa ) . Thanks in advance, wishes, Dr. Bhushan Bonde Computational Biology, Medicines Discovery and Development, GlaxoSmithKline R&D, UK. <[email protected]> _______________________________________________ Genome maillist - [email protected] https://lists.soe.ucsc.edu/mailman/listinfo/genome
