Annotation means you are marking positions on the genome with information.
If you can determine the locations on the genome where you want to place
information, then you can annotate the genome.  Construct your information
in the format of one of the standard UCSC file formats.  If this is
data for a genome that is newly sequenced, you would need your own
local installation of the genome browser.
http://genomewiki.ucsc.edu/index.php/Building_a_new_genome_database

--Hiram

±èÀ¯¸° wrote:
> Hi there,
> 
> I was wondering how do you guys annotate genome from sequencing data?
> I recently obtained sequencing data as a bunch of un-organized reads, that
> we have to align and then annotate. I'd like to use MySQL for annotation
> from the UCSC file refgene, do you have any advice for the annotation part
> ?
> 
> Best,
> M.C.
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