Good morning, I habe been using BLAT V34 in local recently, to do dna against dna alignement (option t=dna and q=dna)
When I compare the results with the one obtained on the UCSC Blat page for the same sequence, only the best hit is the same, while all the others hits are different between local and website results. Please find the attached files with the dna sequence used for the Blat, the results from the website, and the results from local blat. Since the sequence is on chromosome 11, the database I use for blat is only this chromosome 11 and the file Homo_sapiens.GRCh37.62.dna.chromosome.11.fa was obtained from Ensembl. I filtered the website blat results so that only chromosome 11 lines appear. I would appreciate your help. Thanks and best regards, Lise Lise Andrieux, PhD Computational Genomics Life Sciences DPT Barcelona Supercomputing Center Barcelona, Catalunya, Spain
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