Hello,

I have two questions that I would really appreciate your help with
answering. 

===========
Firstly,
===========

I am trying to understand the origin of the "super*" coordinates for the
droPer1 and droSec1 genomes available on the UCSC Genome Browser.

For example, in the D. sechellia assembly, I see that all the chromosomes
are prefixed by "super" on the Genome Browser:
http://genome-mirror.bscb.cornell.edu/cgi-bin/hgTracks?hgsid=36382&chromInfo
Page=. However, from Flybase.org, the GFF files, or any coordinate for that
matter on Flybase, is always prefixed by "scaffold" as can be seen from
ftp://flybase.net/genomes/Drosophila_sechellia/current/gff/. 
Why is this? How were the conversion done from "scaffold" into "super"
coordinates? I'm trying to convert the flybase genes reported in the GFF
files into a file that I can upload to the browser to see the flybase
annotated genes, non-coding RNAs, etc. however this clash of coordinate
names is causing much problems.

I should note that I looked in all the older revisions of the Flybase GFF
files and still I see no "super" prefixed coordinates. I hope I'm not
looking at the wrong flybase GFF files.

The same observation was made in the droPer1 reference assembly. 

=============
Secondly, I've noticed that D. willistoni reference assembly is not
available on the UCSC Genome Browser. Why is this?
 
I've added this genome to the Cornell mirror using the droWil1.fa file
downloaded/available from the UCSC browser. The added genome can be viewed
here:
http://genome-mirror.bscb.cornell.edu/cgi-bin/hgGateway?hgsid=36387&clade=in
sect&org=D.+willistoni&db=0 

On a similar note to the first point above, I've observed that the
coordinates are prefixed with "scaffold" on the browser, but flybase reports
coordinates prefixed with "scf2_":
ftp://flybase.net/genomes/Drosophila_willistoni/current/gff/. 


Thanks,
Jaaved


--
Jaaved Mohammed,
Ph.D. Student of Computational Biology 
Tri-Institutional Training Program in Computational Biology and Medicine 
(Cornell University - Ithaca, Weill Cornell Medical College, and Memorial
Sloan-Kettering Cancer Center)





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