Steve,

Thank you for your quick response!

So when I intersect the conservation table with the USCS genes, what part of
that new table is related to conservation? I do not understand.

I was wondering if there was a more efficient way to acquire a conservation
number related to each gene (sum of logs, average, or something similar)
that is a bit less complicated? After trying what you mentioned I saw that
each gene has hundreds of log scores, making this a bit difficult.

Thanks agan,

Libia
On Oct 12, 2011 2:51 PM, "Steve Heitner" <[email protected]> wrote:

> Hi, Libia.
>
> Intersection results are always in terms of the first table, so if you're
> looking for information regarding conservation, you should start with the
> conservation table and then intersect it with UCSC Genes.  When you create
> the intersection in this order, the last column will be the conservation
> score (log odds score).
>
> As an aside, if you were using the identifiers function to define your
> genes/regions, you will now have to use the define regions button. Note
> that
> you can only define 1,000 regions this way - you will need to use a custom
> track if you have more genes than that. Please write back if you need
> assistance with this.
>
> Please contact us again at [email protected] if you have any further
> questions.
>
> ---
> Steve Heitner
> UCSC Genome Bioinformatics Group
>
> -----Original Message-----
> From: [email protected] [mailto:[email protected]] On
> Behalf Of Libia Pava
> Sent: Tuesday, October 11, 2011 9:47 PM
> To: [email protected]
> Subject: [Genome] Log odds score for any conserved element in a gene
>
> Hello,
>
> I'm trying to collect the log odds score for any conserved element in a
> gene
> by intersecting two tables but I'm unable to get an actual log score.
>
> After selecting the chromosomal position of the genes I want I go to
> Tables.
> Then Under gene and Gene Prediction Tracks, UCSC Genes, I intersect the
> position I want with phastConsElements46wayPlacental, but the excel file I
> gather has no log odds scores.
>
> The BED table shows me the number of exons, the number of base pairs in
> each
> exon, and also some values which I'm unable to interpret that are related
> to
> the number of exons. Are these values the phastCon score presenting the
> exons of each gene? If so, why is the first number always 0?
>
> Is this the correct way of gathering the data I want (a conserve score for
> each gene), if so why am I not getting a score? If I'm doing this wrong I
> would appreciate any feedback on how to do it better.
>
>
> Thanks,
>
> Libia
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