Hi Elham, Please take a look at some of our training materials to help answer your questions: http://genome.ucsc.edu/training.html
Also see our In-Silico PCR tool: http://genome.ucsc.edu/cgi-bin/hgPcr If you have additional questions after reviewing the training material, please contact us again at [email protected]. --- Luvina Guruvadoo UCSC Genome Bioinformatics Group On 10/13/2011 7:39 AM, Elham Mohammadi wrote: > Hi, > Could you please tell me step by step what to do to get to NOS3 (eNOS) > chromosome region, the part that codes for the protein (has a ref Seq), and > then mark the 1KB upstream of the first ATG (methionine, that is used in > translation to protein), and then make a primer for PCR sequencing. > It would be good to be able to do the reverse--design a primer way downstream > of the end of the gene and then try to PCR into somewhere in the middle or > even beginning of the gene. > Thank you. Elham > _______________________________________________ > Genome maillist - [email protected] > https://lists.soe.ucsc.edu/mailman/listinfo/genome _______________________________________________ Genome maillist - [email protected] https://lists.soe.ucsc.edu/mailman/listinfo/genome
