Hi Asaf,

To find out which genbank or refseq entries didn't align by looking in the
gbStatus table. The field of interest to you would be numAligns with the
value of 0. Here is a link to the schema for the hg19 gbStatus table:

http://genome.ucsc.edu/cgi-bin/hgTables?db=hg19&hgta_doSchemaDb=hg19&hgta_doSchemaTable=gbStatus


You can either use the table browser (
http://genome.ucsc.edu/cgi-bin/hgTables) to extract this information or if
you are familiar with mySQL you can use our public mySQL server:

http://genome.ucsc.edu/FAQ/FAQdownloads.html#download29

Here is an example mySQL query:

select count(*) from gbStatus where srcDb="GenBank" and type="mRNA" and
orgCat="native" and numAligns=0;


Hope this helps you get started. If you have further questions, please
contact the mailing list: [email protected].

Vanessa Kirkup Swing
UCSC Genome Bioinformatics Group





---------- Forwarded message ----------
From: Asaf Levy <[email protected]>
Date: Mon, Oct 31, 2011 at 7:21 AM
Subject: Re: [Genome] Unaligned transcripts
To: [email protected]


Hi,
I asked the question below 4 days ago and I did not get a reply.
Do you hold a file of the sequences of unaligned human ESTs and cDNAs?

Regards,
Asaf

On Thu, Oct 27, 2011 at 11:07 PM, Asaf Levy <[email protected]> wrote:

> Dear Sir/Madam,
> Let's start by admitting that I am a great fan of the UCSC genome browser
> for the last 6 years...
>
> Since I know you guys download ESTs/mRNAs routinely and align these to the
> human genomes, I'd like to know if it's possible to get from you a file of
> all those transcript accessions or sequences + metadata (e.g: tissues of
> origin) that you failed to align to the human genome.
>
> Regards,
> Asaf
>
> --
> Asaf Levy, PhD student
> Rotem Sorek Lab
> Weizmann Institute of Science
> Rehovot 76100, Israel
> Tel: +972-8-9346344
> Cell: +972-54-7290026
> [email protected]
> [email protected]
>



--
Asaf Levy, PhD student
Rotem Sorek Lab
Weizmann Institute of Science
Rehovot 76100, Israel
Tel: +972-8-9346344
Cell: +972-54-7290026
[email protected]
[email protected]
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