Hi Nikita,

Please see this Frequently Asked Question (FAQ) for more information on 
how to retrieve promoter regions:
http://genome.ucsc.edu/FAQ/FAQdownloads.html#download18

Also feel free to search the Genome mailing list archives by visiting 
our home page and clicking on "Contact Us" 
(http://genome.ucsc.edu/contacts.html), or email us again at 
[email protected] if you have any further questions.

---
Luvina Guruvadoo
UCSC Genome Bioinformatics Group


On 11/17/2011 8:51 PM, Nikita Pednekar wrote:
> Sir,
>
> Below are the step I am using…
>
> *******************************
>
> Clicking on genomes
>
> Selecting Human
>
> Entering gene symbol in ‘gene’ textfield. Click submit
>
> Clicking on first cartoon of gene (UCSC gene based on RefSeq, UniProt,
> GenBank, CCDS and comparative genomics)
>
> In ‘sequence and links to tools&  database’ , click on ‘Genomic sequence
> (chr…………)
>
> Entered 700 in  ‘Promoter/upstream’ and 300 in ‘Downstream’. Rest deselect.
> Click submit.
>
> Got seq in FASTA format.
>
> *******************************
>
>   However, the downstream 300bp seem to be incorrect in all the cases.
> I would like to know how to extract 300 bases downstream of TSS using UCSC
> genome.
>
> Regards,
> Nikita Pednekar.
> _______________________________________________
> Genome maillist  -  [email protected]
> https://lists.soe.ucsc.edu/mailman/listinfo/genome

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