Hi Diego,

This is beyond the scope of the help we are able to provide via the 
Genome Browser mailing list.  I suggest finding a collaborator familiar 
with shell scripting to help you further.  Good luck with your research!

--
Brooke Rhead
UCSC Genome Bioinformatics Group


On 12/2/11 2:46 PM, Diego F. Pereira wrote:
> Good afternoon,
>
> I'd been trying to figure out how to build a simple script to query several
> intervals at once using bigWigToWig and print the results into a file.
>
> For example:
>
> Instead of having the following lines that print separate files, ...
>
> bigWigToWig -chrom=chr1 -start=1000 -end=1010
> http://hgdownload.cse.ucsc.edu/gbdb/hg19/bbi/wgEncodeBuOrchidV1.bigWig
> qry1.txt
> bigWigToWig -chrom=chr2 -start=2000 -end=2010
> http://hgdownload.cse.ucsc.edu/gbdb/hg19/bbi/wgEncodeBuOrchidV1.bigWig
> qry2.txt
> bigWigToWig -chrom=chr3 -start=3000 -end=3010
> http://hgdownload.cse.ucsc.edu/gbdb/hg19/bbi/wgEncodeBuOrchidV1.bigWig
> qry3.txt
>
> ... I would like to have the same queries in a single script that print into
> a single file.
>
> Many thanks again,
>
> Regards,
>
> Diego
>
> -----Original Message-----
> From: Hiram Clawson [mailto:[email protected]]
> Sent: Thursday, December 01, 2011 7:26 PM
> To: Diego Pereira
> Cc: [email protected]
> Subject: Re: [Genome] Hg19 tables
>
> You need to look where there is actual data.  You can find locations for
> data by looking at the tracks in the genome browser.  Or asking for a larger
> interval:
>
> $ bigWigToWig -chrom=chr1 -start=1000 -end=10010
> http://hgdownload.cse.ucsc.edu/gbdb/hg19/bbi/wgEncodeBuOrchidV1.bigWig
> stdout variableStep chrom=chr1 span=1
> 10004   0.4125
> 10005   -0.18625
> 10006   0.11225
> 10007   0.26725
> 10008   -0.06475
> 10009   0.11
> 10010   0.4125
>
> $ bigWigToBedGraph -chrom=chr1 -start=1000 -end=10010
> http://hgdownload.cse.ucsc.edu/gbdb/hg19/bbi/wgEncodeBuOrchidV1.bigWig
> stdout
> chr1    10003   10004   0.4125
> chr1    10004   10005   -0.18625
> chr1    10005   10006   0.11225
> chr1    10006   10007   0.26725
> chr1    10007   10008   -0.06475
> chr1    10008   10009   0.11
> chr1    10009   10010   0.4125
>
> $ bigWigInfo -chroms
> http://hgdownload.cse.ucsc.edu/gbdb/hg19/bbi/wgEncodeBuOrchidV1.bigWig
> version: 4
> isCompressed: yes
> isSwapped: 0
> primaryDataSize: 14,289,996,669
> primaryIndexSize: 89,736,320
> zoomLevels: 10
> chromCount: 24
>           chr1 0 249250621
>           chr10 1 135534747
>           chr11 2 135006516
>           chr12 3 133851895
>           chr13 4 115169878
>           chr14 5 107349540
>           chr15 6 102531392
>           chr16 7 90354753
>           chr17 8 81195210
>           chr18 9 78077248
>           chr19 10 59128983
>           chr2 11 243199373
>           chr20 12 63025520
>           chr21 13 48129895
>           chr22 14 51304566
>           chr3 15 198022430
>           chr4 16 191154276
>           chr5 17 180915260
>           chr6 18 171115067
>           chr7 19 159138663
>           chr8 20 146364022
>           chr9 21 141213431
>           chrX 22 155270560
>           chrY 23 59373566
> basesCovered: 2,861,325,143
> mean: 0.265295
> min: -0.790750
> max: 1.644750
> std: 0.480838
>
> $ bigWigSummary
> http://hgdownload.cse.ucsc.edu/gbdb/hg19/bbi/wgEncodeBuOrchidV1.bigWig chr1
> 1000 10010 1
> 0.384723
>
> Diego Pereira wrote:
>> Good afternoon Hiram,
>>
>> I tried the following and some variations but I couldn't make it work.
>>
>> bigWigToWig
>> http://hgdownload.cse.ucsc.edu/gbdb/hg19/bbi/wgEncodeBuOrchidV1.bigWig
>> -chrom=chr1
>> -start=1000 -end=1010
>>
>> May I ask you to write it down correctly?
>>
>> Many thanks,
>
> _______________________________________________
> Genome maillist  -  [email protected]
> https://lists.soe.ucsc.edu/mailman/listinfo/genome
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