Hi,
I have a problem about determining gene structure in pig gene genome. Generally
speaking, we considered a sequence aligned to the genome if hits that have >
95% identity across 90% of their length. But when using BLAT, some of my
sequence had a mutiposition,most of which had two or three positions that meet
the above criterion(some even have both 100% identity and 100% length). So how
should I define the position? Should I always take the first one or take both?
Thanks a lot!
Best regards！
weimin zhao
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