Hello, Frank, We have a Restriction Enzyme track in the top track group (Mapping and Sequencing Tracks) below the main Browser graphic. If you click on the link, you will find a configuration page that allows you to add just the enzymes of interest.
As you will read in the explanatory text on that page, the enzymes you cite will not be displayed for windows < 20 kb because of the density of the display. Browsing around the genome with a 20 kb window will give you a rough idea of the extent of digestion, however. If you wish a more complete sense of the site density, you will need to do some processing on your own. The RE track is computed on the fly for display in the Browser and there is no table of enzyme sites to download. The following previously answered question gives some information on how you might get a complete list of the sites for your enzymes. https://lists.soe.ucsc.edu/pipermail/genome/2006-October/011861.html best wishes, and thanks for being Browser user. --b0b kuhn ucsc genome bioinformatics group On 2/10/2012 10:17 AM, Martiniuk, Frank wrote: > Dear UCSC, > > I am looking for a list of restriction sites and number of sites in the > human genome. I want to digest a sample that contains human DNA with some > mycobacterial DNA. Enzymes SWAI; PMEI and PACI very rarely cut the > mycobacterial genome. I need to know if any or all of these 3 enzymes will > fragment the human DNA leaving large mycobacterial fragments. Can you help. > > Frank Martiniuk > NYU School of Medicine > > > ------------------------------------------------------------ > This email message, including any attachments, is for the sole use of the > intended recipient(s) and may contain information that is proprietary, > confidential, and exempt from disclosure under applicable law. Any > unauthorized review, use, disclosure, or distribution is prohibited. If you > have received this email in error please notify the sender by return email > and delete the original message. Please note, the recipient should check this > email and any attachments for the presence of viruses. The organization > accepts no liability for any damage caused by any virus transmitted by this > email. > ================================= > > > _______________________________________________ > Genome maillist - [email protected] > https://lists.soe.ucsc.edu/mailman/listinfo/genome _______________________________________________ Genome maillist - [email protected] https://lists.soe.ucsc.edu/mailman/listinfo/genome
