Hello, We're potentially interested in providing some tracks to include in the public Zebrafish Genome Browser. Our lab (headed by Antonio Giraldez, Yale University Dept. of Genetics) has generated a set of ribosome profiling experiments for early Zebrafish embryos, which we feel may be of great benefit to the Zebrafish research community. Ribosome profiling quantifies mRNAs bound by ribosomes, using high throughput sequencing, and thus is a measure for translational activity; this method was pioneered by Ingolia et al, 2009 (Science, DOI: 10.1126/science.1168978). We envision providing Wiggle tracks similar to the U Mass CHiP-Seq data that show ribosome occupancy levels, as well as tracks that show input RNA seq signal, over 3 timepoints.
We'd appreciate it if you could provide us information about how to begin this process, any sort of guidelines that you would need us to follow, and the timeline for submission and subsequent release of the data that is typical. These data are in support of a publication that is currently under re-review, so we anticipate wanting to make these data public as soon as possible after acceptance. thanks much, Miler Lee _______________________________________________ Genome maillist - [email protected] https://lists.soe.ucsc.edu/mailman/listinfo/genome
