Hello, Olga.

I'm not certain what format you're using to enter your IDs.  If you're using
the RefSeq ID (e.g., NM_020706 which is unique), I'm not certain why you
would be seeing a disproportionate number of results to IDs.  If you're
using the gene name (e.g., SCAF4 which has several transcript variants), I
can understand why you are seeing a disproportionate number of results to
IDs.

If this does not answer the question for you, could you please send the list
of IDs that you are using so I can attempt to recreate the problem?

Please contact us again at [email protected] if you have any further
questions.

---
Steve Heitner
UCSC Genome Bioinformatics Group

-----Original Message-----
From: [email protected] [mailto:[email protected]] On
Behalf Of Olga Müller
Sent: Monday, May 14, 2012 1:11 AM
To: [email protected]
Subject: Re: [Genome] Extract 3' UTR sequence with refSeq

Hello Vanessa, 

 

I have a question concerning the extract of the 3‘ UTR sequences with the
UCSC-software.

 

I want to get the 3‘ UTR sequences of some genes. As an input I used the
Probe Set IDs (and also once with the Transcript IDs) I got from an
Affymetrix expression analysis.

 

When I for example used 200 Transcript IDs as the input, I received UTRs for
more than 200 IDs. And there also not 100% homologous with the input-IDs.

 

What could be the reason for that?

 

I pasted the Input data following this link:

https://lists.soe.ucsc.edu/pipermail/genome/2011-March/025259.html

 

thank you very much in advance.

 

Olga

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