Dear UCSC team,

regarding my original email from Thu Apr 26 01:17:46 PDT 2012, subject:
[Genome] kgXref, rn4.

Could I please have a comment on how to connect rn4 RefSeq to Gene Symbol
and description? I mean RefSeq, *not* RGD!

I want to make a tab-separated table with entries like this:
# RefSeq id       Symbol  Description
NM_001107116.1    Pcp2    Rattus norvegicus Purkinje cell protein 2 (Pcp2),
mRNA.

I got an answer from Luvina on Apr 27. However that answer was with respect
to RGD, but I need to connect RefSeq. We then got sidetrack with some
issues regarding the RGD tables, so herewith I just wanted to come back to
the original question and would be happy if you could help me with this.
Thank you.

Anton


On Wed, May 30, 2012 at 5:52 AM, Steve Heitner <[email protected]> wrote:

> Hello, Anton.
>
> The purpose of the rgdGene2Xref table is to combine the contents of several
> of the supporting tables, but you are correct that there appears to be some
> disagreement between the various fields of rgdGene2Xref.  Thank you for
> bringing this to our attention.  We will certainly look into this issue.
>
> The best solution here would be to link to the individual tables that
> contain the information you are interested in.  Based on your description
> of
> your desired output, follow Luvina's previous instructions, but in the step
> where she instructed you to link to regGene2Xref, you will link to
> rgdGene2ToDescription, rgdGene2ToRefSeq and rgdGene2ToSymbol.
>
> For your output, you will want to select rn4.rgdGene2.name,
> rn4.rgdGene2ToDescription.value, rn4.rgdGene2ToRefSeq.value and
> rn4.rgdGene2ToSymbol.geneSymbol.  You also mentioned that you would like
> coordinates included in your output.  There are several coordinate fields
> in
> the rn4.rgdGene2 table.  Select the fields appropriate to your needs.
>
> Please contact us again at [email protected] if you have any further
> questions.
>
> ---
> Steve Heitner
> UCSC Genome Bioinformatics Group
>
> -----Original Message-----
> From: [email protected] [mailto:[email protected]] On
> Behalf Of Anton Kratz
> Sent: Monday, May 28, 2012 10:00 PM
> To: UCSC Genome Browser Mailing List
> Subject: [Genome] [info and infoType don't match] Re: kgXref, rn4
>
> Dear UCSC team,
>
> thank you for your answer; I have a follow-up question.
>
> I followed your instructions to generate a table with these three fields:
> #rn4.rgdGene2.name    rn4.rgdGene2Xref.infoType    rn4.rgdGene2Xref.info
>
> How is one supposed to make the correct relation between the
> comma-separated
> elements in rn4.rgdGene2Xref.infoType and rn4.rgdGene2Xref.info?
>
> I believe the idea is that the entries for the infoType and info fields are
> themselves comma-separated fields, and are in the same order from left to
> right?! But this is not actually the case (examples see below).
>
> I want to parse this data with my own Perl scripts.
>
> I do not understand how one can (in a Perl or Python script) figure out
> from
> this data which of the entries in rn4.rgdGene2Xref.infoType belongs to
> which
> of the entries in rn4.rgdGene2Xref.info.
>
> For example, the first line:
>
> RGD:1565877    GeneID,ID,Name,gene,    499014,RGD1565877,Zdhhc14,
>
> The field "infoType" has four entries, "info" has three entries.
>
> Or this entry:
>
> RGD:1303141    GeneID,ID,Name,Note,gene,
> 308291,Phactr2,RGD1303141,Phactr2,member of a family of proteins that bind
> protein phosphatase 1 and cytoplasmic actin%3B may play a role in
> regulation
> of the actin cytoskeleton,Phactr2,
>
> In this case the comma-separated fields seem to be mixed up:
>
> GeneID 308291
> ID     Phactr2
> Name   RGD1303141
> Note   Phactr2
> gene   member of a family of proteins that bind protein [...]
> ???    Phactr2
>
> info and infoType do not match, and I get the actual Gene Symbol three
> times...
>
> Could you please explain how to parse a table, which has been generated as
> described in the previous emails (please see below)?
>
> What I want to generate is a table with RGD-Gene ID and coordinates,
> Protein
> encoded, Description in TAB-separated fields so that I can parse this from
> a
> script.
>
> best regards,
> Anton
>
>
> On Fri, Apr 27, 2012 at 3:00 AM, Luvina Guruvadoo
> <[email protected]>wrote:
>
> > Hi Anton,
> >
> > The rgdGene2Xref table should provide the information you are looking
> for.
> > In the Table Browser, make the following selections:
> >
> > track: RGD Genes
> > table: rgdGene2
> > output format: selected fields from primary and related tables
> >
> > Click 'get output'. Scroll down and select rgdGene2Xref from the
> > Linked Tables section and click 'Allow Selection From Checked Tables".
> > From here, you can select fields such as infoType and info from
> rn4.rgdGene2Xref.
> >
> > I hope this helps. Please contact us again at [email protected] if
> > you have any further questions.
> >
> > ---
> > Luvina Guruvadoo
> > UCSC Genome Bioinformatics Group
> >
> >
> >
> > On 4/26/2012 1:17 AM, Anton Kratz wrote:
> >
> >> Dear UCSC team,
> >>
> >> I am trying to make a table that contains RefSeq IDs and various
> >> other information.
> >>
> >> I can not find the table kgXref anymore, when I select RefSeq in the
> >> table browser.
> >>
> >> I assume that RefSeq has been superseded by RGD, but there is a lot
> >> of guessing involved regarding the way the tables are linked.
> >>
> >> Could you please let me know about the relevant changes?
> >>
> >> I'd like to make a table with RefSeq pointing to gene symbol,
> >> description and maybe other type of info.
> >>
> >> thanks,
> >> Anton
> >> ______________________________**_________________
> >> Genome maillist  -  [email protected]
> >> https://lists.soe.ucsc.edu/**mailman/listinfo/genome<https://lists.so
> >> e.ucsc.edu/mailman/listinfo/genome>
> >>
> >
> >
> _______________________________________________
> Genome maillist  -  [email protected]
> https://lists.soe.ucsc.edu/mailman/listinfo/genome
>
>
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