Poster's note : Relevant to DAC. Received much media coverage. EG Science Daily at http://www.sciencedaily.com/releases/2015/10/151022141716.htm,
http://scripts.iucr.org/cgi-bin/paper?S1399004715012183 Acta Cryst. (2015). D71, 1745-1756 doi:10.1107/S1399004715012183 BUY ARTICLE Structural and biophysical characterization of the -carbonic anhydrase from the gammaproteobacteriumThiomicrospira crunogena XCL-2: insights into engineering thermostable enzymes for CO2 sequestration N. A. Díaz-Torres, B. P. Mahon, C. D. Boone, M. A. Pinard, C. Tu, R. Ng, M. Agbandje-McKenna, D. Silverman, K. Scott and R. McKenna Biocatalytic CO2 sequestration to reduce greenhouse-gas emissions from industrial processes is an active area of research. Carbonic anhydrases (CAs) are attractive enzymes for this process. However, the most active CAs display limited thermal and pH stability, making them less than ideal. As a result, there is an ongoing effort to engineer and/or find a thermostable CA to fulfill these needs. Here, the kinetic and thermal characterization is presented of an -CA recently discovered in the mesophilic hydrothermal vent-isolate extremophile Thiomicrospira crunogena XCL-2 (TcruCA), which has a significantly higher thermostability compared with human CA II (melting temperature of 71.9°C versus 59.5°C, respectively) but with a tenfold decrease in the catalytic efficiency. The X-ray crystallographic structure of the dimeric TcruCA shows that it has a highly conserved yet compact structure compared with other -CAs. In addition, TcruCA contains an intramolecular disulfide bond that stabilizes the enzyme. These features are thought to contribute significantly to the thermostability and pH stability of the enzyme and may be exploited to engineer -CAs for applications in industrial CO2 sequestration. Keywords: Thiomicrospira crunogena XCL-2; -carbonic anhydrase; protein thermostability; biocatalyst; extremophile; CO2 sequestration. -- You received this message because you are subscribed to the Google Groups "geoengineering" group. To unsubscribe from this group and stop receiving emails from it, send an email to [email protected]. To post to this group, send email to [email protected]. Visit this group at http://groups.google.com/group/geoengineering. For more options, visit https://groups.google.com/d/optout.
