On 11/6/14 4:53 PM, Chris Papamitrou wrote:
I am trying to perform a simulation regarding the effect that hydration has
on tensile response for my system.  When i looked into the umbrella
sampling tutorial it said to create a start file using the

pdb2gmx -f input.pdb -ignh -ter -o complex.gro

command and was wondering what effect that the -ignh option had on my
simulated results?  do the hydrogens
not get accounted for in this simulation, and if so, how are the
atomic bonds in which the hydrogens are
supposed to be located treated in terms of replacing those hydrogens?
does this mean i am doing a
united atom model ?


Start with pdb2gmx -h. The -ignh has a very simple purpose, and it's used mostly for working around nomenclature issues.

Whether or not you use a united-atom force field is entirely up to you, based on what you choose.

-Justin

--
==================================================

Justin A. Lemkul, Ph.D.
Ruth L. Kirschstein NRSA Postdoctoral Fellow

Department of Pharmaceutical Sciences
School of Pharmacy
Health Sciences Facility II, Room 629
University of Maryland, Baltimore
20 Penn St.
Baltimore, MD 21201

[email protected] | (410) 706-7441
http://mackerell.umaryland.edu/~jalemkul

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