Hi Justin Thanks for your valuable advice. I had a misconception that in GROMACS Just adding NA and CL doesn't produce the effect of salt concentration. It arises because most of the paper I have read about effect of salt concentration on protein or DNA the structure of the bio molecule doesn't shows any significant change (this may be also because those protein doesn't change much with salt concentration). I read your recent paper where you used "Drude Polarizable Force Fields" which can't be used in GROMACS.
As you said that it depends on force field so I am just curious to know which force field and water model one should use for simulating a system (preferably protein in water) at different salt concentration to see the effect of salt concentration on the structure of the protein. Thanks a lot for your time and advice. -- Tushar Ranjan Moharana B. Tech, NIT Warangal Ph D Student, CCMB -- Gromacs Users mailing list * Please search the archive at http://www.gromacs.org/Support/Mailing_Lists/GMX-Users_List before posting! * Can't post? Read http://www.gromacs.org/Support/Mailing_Lists * For (un)subscribe requests visit https://maillist.sys.kth.se/mailman/listinfo/gromacs.org_gmx-users or send a mail to gmx-users-requ...@gromacs.org.