Levi,

If you are comparing between the two concentrations, your cutoffs (i.e. the 
defined measures) should not be changed. If you are trying to determine if you 
can re-construct the averages from the bulk solution from the 200mM NaCl 
solution than you will need to sweep through a variety of cutoffs and see which 
one matches best; though will increasing salt, you can imagine that the 
electrostatics that lead to the formation of salt-bridges will be screened 
(weakend) and you may find less of them.

-Micholas

===================
Micholas Dean Smith, PhD.
Post-doctoral Research Associate
University of Tennessee/Oak Ridge National Laboratory
Center for Molecular Biophysics

________________________________________
From: [email protected] 
<[email protected]> on behalf of LW 
<[email protected]>
Sent: Wednesday, August 05, 2015 8:44 PM
To: [email protected]
Subject: [gmx-users] how salt concentration influence the cutoff distances for 
hbond and salt bridges

Dear all,

I ran two simulations of my protein, one in the absence of NaCl, and
another in the presence of 200 mM NaCl. With the simulated trajectories, I
am not certain how salt concentration influence the cutoff distances for
hbond and salt bridges. Any help is grately appreciated.

Best regards,

Levi
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