I would mildly disagree with Justin on this one. Although Charmm should ideally 
be used with TIP3P (as Justin noted being one of the force-field developers and 
all), there have been a number of papers that show that SPC/E is not entirely 
incorrect (see below). What you would need to do is see if there is any 
experimental evidence you can compare to as a sanity check; though, many of the 
papers I cite below do indicate that SPC/E does still get the overall dynamics 
correct even when used with Charmm.

---------------------------------
Citations of interest.

Force-​Field Induced Bias in the Structure of Aβ21-​30: A Comparison of OPLS, 
AMBER, CHARMM, and GROMOS Force-​Fields. DOI: 10.1021/acs.jcim.5b00308

Structure of Aqueous Proline via Parallel Tempering Molecular Dynamics and 
Neutron Diffraction. DOI: 10.1021/jp0714973 

Testing the inter-operability of the CHARMM and SPC/Fw force fields for 
conformational sampling. DOI: 10.1080/08927022.2013.824574  (note* this is an 
SPC/E derivative water model, you milage may vary)

Local Order, Energy, and Mobility of Water Molecules in the Hydration Shell of 
Small Peptides. DOI: 10.1021/jp909090u

Free Energy Landscapes of Alanine Oligopeptides in Rigid-Body and Hybrid Water 
Models. DOI: 10.1021/acs.jpcb.5b02937 

-Micholas

________________________________________
From: [email protected] 
<[email protected]> on behalf of Justin Lemkul 
<[email protected]>
Sent: Monday, December 07, 2015 8:14 PM
To: [email protected]
Subject: Re: [gmx-users] using SPC water with Charmm FF

On 12/7/15 6:59 PM, jhon espinosa wrote:
> Hi allI finished running the 50 ns MD Simulation of a very large system (17 
> Million atoms).It is a capsid in water. I used charmm FF for the protein and 
> spc for the water.I am studying temperature triggered structural 
> transitions.Since Charmm FF is build based on TIP3p water model, are my 
> simulation results wrong?Is there any main issue that I should know?ThanksJohn
>

Sorry to say that is an invalid physical model.  The balance of forces will be
totally wrong.

-Justin

--
==================================================

Justin A. Lemkul, Ph.D.
Ruth L. Kirschstein NRSA Postdoctoral Fellow

Department of Pharmaceutical Sciences
School of Pharmacy
Health Sciences Facility II, Room 629
University of Maryland, Baltimore
20 Penn St.
Baltimore, MD 21201

[email protected] | (410) 706-7441
http://mackerell.umaryland.edu/~jalemkul

==================================================
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