Hi GROMACS Users,

I would like to create a mixed bilayer of DPPC and DOTAP lipids for
research. I usually use charm-gui or insane.py to create a mixed bilayer.
But I could not find DOTAP listed in either of these lists. When I searched
online, I got a mixed bilayer system for DMPC & DPPC lipids and the
parameter file for DOTAP lipid from http://www.softsimu.net/downloads.shtml.

My objective is to create mixed lipid bilayer system with various
percentage of DOTAP lipids. In addition, I have to create a bilayer with
one DOTAP in each layer and remaining DPPC lipids. Can anyone please guide
me to create these lipid bilayers? It would be great if someone could
explain, how to create these mixed bilayers from scratch.

Thanks a lot everyone!,

Nidhin Thomas
University of Houston
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