Hi Amali,

gmx hbond identifies typical acceptor and donor groups by default. In fact, 
analysing non-standard donors/acceptors is a bit underdeveloped. What the 
program basically does, if memory serves me right, is to identify N and O with 
H bound to them, or optionally without H in case of acceptors. If you wish to 
analyse the hbonds separated by residue number you may wan to either run vmx 
bond multiple times with one group being a single residue and the other the 
ligand, or you can run it once with the -hbm and -hbn options and extract the 
information from the resulting matrix.

Kind regards,
Erik


> On 28 Jun 2016, at 08:59, Amali Guruge <[email protected]> wrote:
> 
> Thank you very much for the answer. How we identify residues involving in H
> bonds? In index file do we have to specify them?
> 
> On Tue, Jun 28, 2016 at 11:45 AM, Subhomoi Borkotoky <[email protected]>
> wrote:
> 
>> Hello,
>> 
>> Please use the "hbond"  utility in gromacs.
>> 
>> gmx hbond [-f [<.xtc/.trr/...>]] [-s [<.tpr/.tpb/...>]] [-n
>> [<.ndx>]][-num [<.xvg>]
>> 
>> 
>> On Tue, Jun 28, 2016 at 11:24 AM, Amali Guruge <[email protected]>
>> wrote:
>> 
>>> Dear Gromacs users,
>>> 
>>> I carried out 20 ns long MD simulation for my receptor-ligand complex
>> using
>>> Gromacs software. Now I want to calculate number of hydrogen bonds formed
>>> in the ligand and receptor corresponding to the residue number. Can
>> anyone
>>> help me?
>>> 
>>> 
>>> Thank you.
>>> --
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>> 
>> 
>> 
>> --
>> Yours Sincerely,
>> --------------------------
>> SUBHOMOI BORKOTOKY,
>> Centre for Bioinformatics
>> Pondicherry University
>> Pondicherry,INDIA.
>> 
>> Alternate Email: [email protected]
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