On Fri, Jul 25, 2014 at 01:12:00AM -0700, Witchukorn Phuthong wrote:
> Using Gwyddion, I do the basic leveling, scar removal and fourier noise
> filtration, adjust the contrast and export my AFM image to RGB tiff file
> and the contrast level has the height difference about 5 nm. Then, I would
> like to associate the height and the brightness for further analysis in
> imageJ. I also convert my RGB tiff file to 8-bit grayscale for this
> analysis in ImageJ.
I'm pretty sure ImageJ can read text data matrix. So save the data to
something.txt in Gwyddion and load that into ImageJ.
If you insist on a pixmap image format, use high-depth (16bit) greyscale
PNG. Or OpenEXR. Gwyddion saves the physical scaling info into all of
them, see
http://gwyddion.net/documentation/user-guide-en/pixmap-image-keys.html
In the case of PNG, any image software supporting the standard sCAL and
pCAL chunks should be able to open the image with the right scale
automatically (not sure if this applies to ImageJ or not).
Regards,
Yeti
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