I used a CBG recycler for years.....many years and absolutely loved it! Worked great, excellent recovery (xylene) and minimal maintenance. Great (people) support from the company too. Jeanne
Jeanne Clark HT/MLT (ASCP) Pathology Manager Mission Hospitals Asheville, NC 423-612-1213 --- On Wed, 9/10/08, [EMAIL PROTECTED] <[EMAIL PROTECTED]> wrote: From: [EMAIL PROTECTED] <[EMAIL PROTECTED]> Subject: Histonet Digest, Vol 58, Issue 12 To: [email protected] Date: Wednesday, September 10, 2008, 1:21 PM Send Histonet mailing list submissions to [email protected] To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to [EMAIL PROTECTED] You can reach the person managing the list at [EMAIL PROTECTED] When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Manual Paraffin Embedding (Merced Leiker) 2. Manual Paraffin Embedding (Paula Pierce) 3. Re: Manual Paraffin Embedding (Bryan Llewellyn) 4. Re: Histonet Digest, Vol 58, Issue 8 (Cheryl Crowder) 5. Re: methylene blue (Rene J Buesa) 6. Re: Manual Paraffin Embedding (Rene J Buesa) 7. Re: Manual Paraffin Embedding (Emily Sours) 8. Paraformaldehyde (Mary P. Brownson) 9. RE: Manual Paraffin Embedding (Kemlo Rogerson) 10. Histology Positions (Cheri Miller) 11. Help (RENEE FISHER) 12. please un subscribe (RENEE FISHER) 13. Recyclers (Bauer, Karen) ---------------------------------------------------------------------- Message: 1 Date: Tue, 09 Sep 2008 16:54:43 -0400 From: Merced Leiker <[EMAIL PROTECTED]> Subject: [Histonet] Manual Paraffin Embedding To: [email protected] Message-ID: <[EMAIL PROTECTED]> Content-Type: text/plain; charset=us-ascii; format=flowed Does anyone process and embed tissues manually instead of using automated and expensive equipment? Can you tell me how you do it? Thanks. Merced ------------------------------ Message: 2 Date: Tue, 9 Sep 2008 14:19:57 -0700 (PDT) From: Paula Pierce <[EMAIL PROTECTED]> Subject: [Histonet] Manual Paraffin Embedding To: Merced Leiker <[EMAIL PROTECTED]>, Histonet <[email protected]> Message-ID: <[EMAIL PROTECTED]> Content-Type: text/plain; charset=iso-8859-1 I sometimes still use a paraffin dispenser for really large blocks. It looks like a big coffee maker, like caterers use. You will still need molds. Embedding centers work on the same principle, they just incorporate the melted paraffin bay and dispenser with a cold plate on the side. Paula Pierce ----- Original Message ---- From: Merced Leiker <[EMAIL PROTECTED]> To: [email protected] Sent: Tuesday, September 9, 2008 3:54:43 PM Subject: [Histonet] Manual Paraffin Embedding Does anyone process and embed tissues manually instead of using automated and expensive equipment? Can you tell me how you do it? Thanks. Merced _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 3 Date: Tue, 9 Sep 2008 15:21:02 -0700 From: "Bryan Llewellyn" <[EMAIL PROTECTED]> Subject: Re: [Histonet] Manual Paraffin Embedding To: <[email protected]> Message-ID: <[EMAIL PROTECTED]> Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=response I don't do this anymore, nor for 40 years now, but this is what we used to do aeons ago. 1. Fix in 10% NBF for 48 hours. 2 Rinse off excess with tap water for 1 minute. 3. Select pieces of tissue with maximum dimensions of 2cm x 1.5 cm x 0.3 cm. 4. Place into cassettes if you have them, then into a large container. If you do not have cassettes, place into small jars, each case in a different jar. Place a label in each cassette or each jar with the case ID. 5. Cover the tissue or cassettes with 70% ethanol, methylated spirits or isopropanol, agitate gently. Leave overnight. 6. Next morning, replace the 70& alcohol with 85% alcohol, leave for the day, agitating gently periodically. 7. Before leaving in the evening, replace the alcohol with 95% alcohol, agitate gently and leave overnight. 8. Next morning, replace the alcohol with 100% alcohol, gently agitate periodically. Repeat at noon and before you 9. leave for the evening, gently agitating. 10. Next morning, replace the alcohol with clearant, preferably xylene or toluene. Leave for one hour, gently agitating periodically. 11. Repeat the clearant twice more, agitate gently. 12. Place into premelted paraffin wax for 1 hour at 65C. Check periodically and when all congealed wax has remelted, agitate gently. 13. Repeat at least twice more, preferably under vacuum - not too strong. Some technologists used to leave the final change overnight. Doing so doesn't do much harm and improves penetration. Agitation can't be done under vacuum, so release the vacuum periodically, agitate and reapply it. 14. Block out into molds. (If you do not have molds, use a metal - tinned steel or aluminum - lid with a depth of 1cm. LIGHTLY coat it with glycerol first.) Place a thin (3mm) layer of hot wax in the mold and place the tissue, with the surface to be sectioned down, into it. Top up the mold with wax so there is at least 2-3 mm wax over the top of the tissue. Put the ID label conspiciously next to the tissue. Do NOT block out more than one tissue or case without placing the ID labels, this WILL lead to serious identification errors. Do all this by the oven and keep the door closed as much as possible. Work fast so that the wax around the tissues does not begin to congeal as that causes problems during sectioning and floating out. You must get the wax around the tissue and the wax in the mold to blend completely, so if the wax congeals around the tissue, leave it to remelt before blocking out. Many of us used to keep a bunsen burner alight and flame the top of the molds to keep the wax molten - a practice probably considered unsafe now. 15. Allow the wax in the mold to skin over thoroughly, then GENTLY lower into cold tap water to cool. 16. When completely cold and solid, use a heavy knife to score and trim the wax blocks. 17. To section, melt the trimmed block onto the block holder. Bryan Llewellyn ----- Original Message ----- From: "Merced Leiker" <[EMAIL PROTECTED]> To: <[email protected]> Sent: Tuesday, September 09, 2008 1:54 PM Subject: [Histonet] Manual Paraffin Embedding > Does anyone process and embed tissues manually instead of using automated > and expensive equipment? Can you tell me how you do it? Thanks. > > Merced > > > _______________________________________________ > Histonet mailing list > [email protected] > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 4 Date: Tue, 09 Sep 2008 17:54:41 -0500 From: "Cheryl Crowder" <[EMAIL PROTECTED]> Subject: [Histonet] Re: Histonet Digest, Vol 58, Issue 8 To: [email protected] Message-ID: <[EMAIL PROTECTED]> Content-Type: text/plain; charset="us-ascii" Maxim - You sent me an e-mail in August. I replied several times, but the e-mail did not go through. Is your address complete or is there another I can use? Cheryl Crowder, BA, HTL(ASCP) Chief Technologist Anatomic Pathology Department of Pathobiological Sciences School of Veterinary Medicine Louisiana State University Skip Bertman Drive Baton Rouge, LA 70803 225-578-9734 FAX: 225-578-9720 -----Original Message----- From: [EMAIL PROTECTED] To: [email protected] Date: Sun, 07 Sep 2008 12:02:56 -0500 Subject: Histonet Digest, Vol 58, Issue 8 Send Histonet mailing list submissions to [email protected] To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet [http://lists.utsouthwestern.edu/mailman/listinfo/histonet] or, via email, send a message with subject or body 'help' to [EMAIL PROTECTED] You can reach the person managing the list at [EMAIL PROTECTED] When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. RE: reagent question ([EMAIL PROTECTED]) ---------------------------------------------------------------------- Message: 1 Date: Sat, 6 Sep 2008 23:38:42 +0400 From: [EMAIL PROTECTED] Subject: RE: [Histonet] reagent question To: [EMAIL PROTECTED] Cc: [email protected] Message-ID: <[EMAIL PROTECTED]> Content-Type: text/plain; charset=us-ascii More than one year we uses isopropanol as dehydratant and enjoy for quality of specimens, which processed with this reagent. It is very useful for bone, cartillage, uterus, breast, colon, skin, eyeball and other difficult specimens. Some unpleasant odor of "cat urine" does not disturb to use it in manual processing. We believes, that will be time, when we will have a vacuum processor... For slides we use acetone as H&E as SS. We stain all our slides manually. Maxim Peshkov, Russia, Taganrog. mailto:[EMAIL PROTECTED] ------------------------------ _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet [http://lists.utsouthwestern.edu/mailman/listinfo/histonet] End of Histonet Digest, Vol 58, Issue 8 *************************************** ------------------------------ Message: 5 Date: Wed, 10 Sep 2008 05:00:17 -0700 (PDT) From: Rene J Buesa <[EMAIL PROTECTED]> Subject: Re: [Histonet] methylene blue To: [email protected], "Perry, Margaret" <[EMAIL PROTECTED]> Message-ID: <[EMAIL PROTECTED]> Content-Type: text/plain; charset=iso-8859-1 Methylene blue does not require anything special, this stain never fails. Just hydrate your section, stain with 1% aq. methBlue for 5 minutes, wash and dehydrate QUICKLYLY cover and that is all.<René J. --- On Tue, 9/9/08, Perry, Margaret <[EMAIL PROTECTED]> wrote: From: Perry, Margaret <[EMAIL PROTECTED]> Subject: [Histonet] methylene blue To: [email protected] Date: Tuesday, September 9, 2008, 4:22 PM Help! We have been asked to do a methylene blue stain for Myxosporidia and don't have a procedure. Of course the pathologist wants it quickly. I can't get into the archives. It must be down right now. Can someone help us? Margaret Perry HT (ASCP) IHC Lab Manager Veterinary Science Animal Disease Research and Diagnostic Lab South Dakota State University Box 2175 North Campus Drive Brookings SD 57007 _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 6 Date: Wed, 10 Sep 2008 05:09:14 -0700 (PDT) From: Rene J Buesa <[EMAIL PROTECTED]> Subject: Re: [Histonet] Manual Paraffin Embedding To: [email protected], Merced Leiker <[EMAIL PROTECTED]> Message-ID: <[EMAIL PROTECTED]> Content-Type: text/plain; charset=iso-8859-1 Merced: Processing and embedding tissues manually is still done occasionally here in the US and specially abroad. As a matter of fact 2% us US labs and 14% of foreign labs routinely manually process their tissues. It implies running the tissues through all the dehydration, clearing and infiltration steps to finally prepare the blocks, also manually, using melted paraffin and dispensed in paper molds, or using Leuckhart rectangles. The description would be very long for an e-mail, so my advise is to get a histotechnique book, like Bolles-Lee's "Microtomist Vade-Mecum", or Peter Gray's "The Microtomist's formulary and guide", both are for sale at Amazon.com/books or you may find a copy at the University of Buffalo. René J. --- On Tue, 9/9/08, Merced Leiker <[EMAIL PROTECTED]> wrote: From: Merced Leiker <[EMAIL PROTECTED]> Subject: [Histonet] Manual Paraffin Embedding To: [email protected] Date: Tuesday, September 9, 2008, 4:54 PM Does anyone process and embed tissues manually instead of using automated and expensive equipment? Can you tell me how you do it? Thanks. Merced _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 7 Date: Wed, 10 Sep 2008 09:31:17 -0400 From: "Emily Sours" <[EMAIL PROTECTED]> Subject: Re: [Histonet] Manual Paraffin Embedding To: [email protected] Message-ID: <[EMAIL PROTECTED]> Content-Type: text/plain; charset=ISO-8859-1 We just use melted paraffin in a vacuum oven. The paraffin is kept at 60C in small glass petri dishes and is moved from dish to dish every half hour for about 3 hours. Then you fill a plastic mold halfway, let the paraffin cool until it's hard enough to pour more on without it melting again, but not fully cooled, put in your tissue, cover it with melted paraffin and let it cool overnight. You don't need the vacuum oven, it just gets rid of bubbles in the paraffin. You also need to use heated instruments (we just warm them briefly in an alcohol lamp flame) when transferring the embryo in paraffin. Emily -- An overcivilized people grow complacent and careless and leave the door open for a tribe of fanatical savages, through a mixture of luck, treachery, and the foulest inhumanity, to usurp their place for a few years. -Richard Adams, "Shardik", 1974 ------------------------------ Message: 8 Date: Tue, 9 Sep 2008 14:50:33 -0600 From: "Mary P. Brownson" <[EMAIL PROTECTED]> Subject: [Histonet] Paraformaldehyde To: <[email protected]> Message-ID: <[EMAIL PROTECTED]> Content-Type: text/plain; charset="us-ascii" Hello, I plan to use paraformaldehyde for perfusion of rat brains. My question is; what grade paraformaldehde should I be using? Reagent grade, 95% or other? Or does it not matter? Thank you, Mary Brownson School of Pharmacy University of Wyoming Laramie, WY 82070 [EMAIL PROTECTED] ------------------------------ Message: 9 Date: Wed, 10 Sep 2008 15:10:35 +0100 From: "Kemlo Rogerson" <[EMAIL PROTECTED]> Subject: RE: [Histonet] Manual Paraffin Embedding To: "Merced Leiker" <[EMAIL PROTECTED]>, <[email protected]> Message-ID: <[EMAIL PROTECTED]> Content-Type: text/plain; charset="us-ascii" Does anyone process and embed tissues manually instead of using automated and expensive equipment? Can you tell me how you do it? Thanks. Merced When I was a lad I used to manual process Brains and other stuff. In retrospect I wonder why we use the same processing schedules for disparate organs. We ought to have a kidney schedule and a heart schedule specifically for each organ type. I always knew a tissue was processed adequately as it changed in its translucency. If the was water still present in the tissue you could see its presence in the clearing agent and smell clearing agent in the wax. However you exposed yourself to carcinogenic vapours and I guess that it is right and proper we automated and contained. I wonder if that's why I've grown two heads? Kemlo Rogerson Pathology Manager DD 01934 647057 or extension 3311 Mob 07749 754194; Pager 07659 597107; Don't be afraid to take a big step when one is indicated. You can't cross a chasm in two small jumps. --Buckminster Fuller This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation ------------------------------ Message: 10 Date: Wed, 10 Sep 2008 10:06:56 -0500 From: "Cheri Miller" <[EMAIL PROTECTED]> Subject: [Histonet] Histology Positions To: <[email protected]> Cc: <[EMAIL PROTECTED]> Message-ID: <[EMAIL PROTECTED]> Content-Type: text/plain; charset="us-ascii" Is there anyone looking for a Histotech or a Histology Supervisor in the Omaha, Council Bluffs or Lincoln area?? Let me know. Thanks, Cheri Cheryl Miller HT (ASCP) Histology Supervisor Physicians Laboratory,P.C. Omaha, Ne. 402 738 5052 PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. ------------------------------ Message: 11 Date: Wed, 10 Sep 2008 12:16:54 -0400 From: "RENEE FISHER" <[EMAIL PROTECTED]> Subject: [Histonet] Help To: <[email protected]> Message-ID: <[EMAIL PROTECTED]> Content-Type: text/plain; charset=US-ASCII please un scribe _______________________________________________________________________________________ This email may contain confidential protected health information and/or proprietary information belonging to the sender that is legally privileged under local, state, or federal law. This information is intended only for the use of the individual or individuals who have received this. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of this email is strictly prohibited. If you have received this email in error, please notify the sender immediately to arrange for the disposal of this information. ------------------------------ Message: 12 Date: Wed, 10 Sep 2008 12:19:12 -0400 From: "RENEE FISHER" <[EMAIL PROTECTED]> Subject: [Histonet] please un subscribe To: <[email protected]> Message-ID: <[EMAIL PROTECTED]> Content-Type: text/plain; charset=US-ASCII please un subscribe _______________________________________________________________________________________ This email may contain confidential protected health information and/or proprietary information belonging to the sender that is legally privileged under local, state, or federal law. This information is intended only for the use of the individual or individuals who have received this. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of this email is strictly prohibited. If you have received this email in error, please notify the sender immediately to arrange for the disposal of this information. ------------------------------ Message: 13 Date: Wed, 10 Sep 2008 11:58:09 -0500 From: "Bauer, Karen" <[EMAIL PROTECTED]> Subject: [Histonet] Recyclers To: <[email protected]> Message-ID: <[EMAIL PROTECTED]> Content-Type: text/plain; charset="iso-8859-1" Hello, I'm in the market to demo some recyclers. Any vendors are welcome to contact me personally. I've already contacted CBG and BR Instruments, so vendors from those areas need not reply to this message. Anyone on Histonet that would like to give me their likes and dislikes of certain recyclers, I'm all ears. Thanks much, Karen Karen L. Bauer HT(ASCP) Department of Pathology Histology Section Chief Luther Hospital Eau Claire, WI 715-838-3205 ********************Confidentiality Notice******************** This message is intended for the sole use of the individual and entity to whom it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure or distribution of this email message, including any attachment, is prohibited. If you are not the intended recipient, please advise the sender by reply email and destroy all copies of the original message. Thank you. ------------------------------ _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 58, Issue 12 **************************************** _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
