Dear All, Does anyone have any experience with staining pig endothelium, specifically keeping rings of blood vessels alive?
I am collecting fresh tissue from the slaughterhouse and incubating it with tumour necrosis factor to induce VCAM-1 expression. However, I suspect that the tissue quickly loses viability and this is why I canĀ¹t visualise any VCAM with IHC. Does anyone have any tips for keeping the rings of tissue going/fixing/antigen retrieval? Many thanks Alistair Lindsay MBChB MRCP Tel.: 07989404808 [EMAIL PROTECTED] [EMAIL PROTECTED] _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet